caa a22 4500 475801741 CHVBK 20180406123739.0 cr unu---uuuuu 170329e20000901xx s 000 0 eng 10.1007/s002680010181 doi (NATIONALLICENCE)springer-10.1007/s002680010181 Experimental Study of the Effect of Nitric Oxide Inhibition on Mesenteric Blood Flow and Interleukin-10 Levels with a Lipopolysaccharide Challenge [Elektronische Daten] [Ataç Baykal, Burak Kavuklu, Alper B. Iskit, M. Oguz Guc, Gulsen Hascelik, Iskender Sayek] The septic shock-induced decrease in mesenteric blood flow and release of proinflammatory cytokines are among the major pathophysiologic changes presumed to lead to multiple organ dysfunction syndrome (MODS). Increased nitric oxide (NO) levels are associated with both decreased mesenteric blood flow and positive modulation of proinflammatory cytokine release. In this study we aimed to determine the effect of the timing of the inhibition of nitric oxide synthase (NOS) on mesenteric blood flow and serum interleukin-10 (IL-10) concentrations during endotoxin shock. A nonspecific NOS inhibitor N G-nitro-l-arginine methyl ester (l-NAME), a specific NOS inhibitor aminoguanidine (AG), or placebo were injected 20 minutes before or 20 minutes after a lipopolysaccharide (LPS) or placebo challenge to Swiss-albino mice, as pretreatment or posttreatment, respectively. At 120 minutes after LPS or placebo injection the mesenteric blood flow was measured, and blood samples from the heart were obtained for IL-10 levels in both groups. Pretreatment and posttreatment with both NOS inhibitors prevented the LPS-induced decrease in mesenteric blood flow. Pretreatment was more effective for this purpose. Pretreatment accentuated the LPS-induced increase in serum IL-10 concentrations, whereas posttreatment had no significant effect. We conclude that the timing of NOS inhibition is important for attenuating some deleterious effects of endotoxin. Société Internationale de Chirurgie, 2000 Baykal Ataç Department of General Surgery, Hacettepe University School of Medicine, Ankara 06100, Turkey aut Kavuklu Burak Department of General Surgery, Hacettepe University School of Medicine, Ankara 06100, Turkey aut Iskit Alper B. Department of Pharmacology, Hacettepe University School of Medicine, Ankara 06100, Turkey aut Guc M. Oguz Department of Pharmacology, Hacettepe University School of Medicine, Ankara 06100, Turkey aut Hascelik Gulsen Department of Microbiology, Hacettepe University School of Medicine, Ankara 06100, Turkey aut Sayek Iskender Department of General Surgery, Hacettepe University School of Medicine, Ankara 06100, Turkey aut World Journal of Surgery Springer-Verlag 24/9(2000-09-01), 1116-1120 0364-2313 24:9<1116 2000 24 268 https://doi.org/10.1007/s002680010181 text/html Onlinezugriff via DOI 1 research-article jats NATIONALLICENCE 856 40 https://doi.org/10.1007/s002680010181 text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Baykal Ataç Department of General Surgery, Hacettepe University School of Medicine, Ankara 06100, Turkey aut NATIONALLICENCE 700 1- Kavuklu Burak Department of General Surgery, Hacettepe University School of Medicine, Ankara 06100, Turkey aut NATIONALLICENCE 700 1- Iskit Alper B. Department of Pharmacology, Hacettepe University School of Medicine, Ankara 06100, Turkey aut NATIONALLICENCE 700 1- Guc M. Oguz Department of Pharmacology, Hacettepe University School of Medicine, Ankara 06100, Turkey aut NATIONALLICENCE 700 1- Hascelik Gulsen Department of Microbiology, Hacettepe University School of Medicine, Ankara 06100, Turkey aut NATIONALLICENCE 700 1- Sayek Iskender Department of General Surgery, Hacettepe University School of Medicine, Ankara 06100, Turkey aut NATIONALLICENCE 773 0- World Journal of Surgery Springer-Verlag 24/9(2000-09-01), 1116-1120 0364-2313 24:9<1116 2000 24 268 Metadata rights reserved Springer special CC-BY-NC licence nationallicence BK010053 XK010053 XK010000 NATIONALLICENCE NATIONALLICENCE NL-springer