caa a22 4500 475802756 CHVBK 20180406123742.0 cr unu---uuuuu 170329e20001001xx s 000 0 eng 10.1007/s002680010201 doi (NATIONALLICENCE)springer-10.1007/s002680010201 New Ultradeformable Drug Carriers for Potential TransdermalApplication of Interleukin-2 and Interferon-a Theoretic and PracticalAspects [Elektronische Daten] [Christian Hofer, Rudolf Hartung, Roland Göbel, Petra Deering, Antonie Lehmer, Jürgen Breul] Abstract : Transfersomes (TFs) are highly deformable hydrophilic lipid vesicles that are able to penetrate the skin barrier spontaneously because of their characteristics. Transfersomes are able to transport noninvasively low- and high-molecular-weight molecules into the body. We describe the formulation and several biologic characteristics of interleukin-2 (IL-2)- and interferon-α (IFNα)-containing TFs. TFs contain natural phosphatidylcholine and sodium cholate. Recombinant human IL-2 and human hybrid IFNα were added to TFs and incubated for 24 hours at 4°C. Immunotransfersomes were isolated from free IL-2 and IFNα by filtration (Centrisart, Sartorius). The biologic activity of immunotransfersomes was measured by a cytotoxic lymphoid line assay for IL-2 and by an A549-encephalomyocarditis virus assay for IFN; concentrations of proteins were determined by the enzyme-linked immunosorbent assay (ELISA). It was possible to incorporate a large amount of IL-2 and IFN in TFs (75-80%), and the incorporated IL-2, and IFN were biologically active. The increased lipid/protein ratio (90.9/1.0) led to a growing probability of association. We were thus able to show that IL-2 and IFN are trapped by transfersomes in a biologically active form and in sufficient concentrations for immunotherapy. In upcoming experiments these IL-2- and IFN-containing TFs will be used for a transdermal approach in the murine RENCA cell line model. by Société Internationale de Chirurgie, 2000 Hofer Christian Urologische Klinik und Poliklinik, Technischen Universität München, Ismaningestrasse 22, 81675 München, Germany, DE aut Hartung Rudolf Urologische Klinik und Poliklinik, Technischen Universität München, Ismaningestrasse 22, 81675 München, Germany, DE aut Göbel Roland Urologische Klinik und Poliklinik, Technischen Universität München, Ismaningestrasse 22, 81675 München, Germany, DE aut Deering Petra Urologische Klinik und Poliklinik, Technischen Universität München, Ismaningestrasse 22, 81675 München, Germany, DE aut Lehmer Antonie Urologische Klinik und Poliklinik, Technischen Universität München, Ismaningestrasse 22, 81675 München, Germany, DE aut Breul Jürgen Urologische Klinik und Poliklinik, Technischen Universität München, Ismaningestrasse 22, 81675 München, Germany, DE aut World Journal of Surgery Springer-Verlag 24/10(2000-10-01), 1187-1189 0364-2313 24:10<1187 2000 24 268 https://doi.org/10.1007/s002680010201 text/html Onlinezugriff via DOI 1 research-article jats NATIONALLICENCE 856 40 https://doi.org/10.1007/s002680010201 text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Hofer Christian Urologische Klinik und Poliklinik, Technischen Universität München, Ismaningestrasse 22, 81675 München, Germany, DE aut NATIONALLICENCE 700 1- Hartung Rudolf Urologische Klinik und Poliklinik, Technischen Universität München, Ismaningestrasse 22, 81675 München, Germany, DE aut NATIONALLICENCE 700 1- Göbel Roland Urologische Klinik und Poliklinik, Technischen Universität München, Ismaningestrasse 22, 81675 München, Germany, DE aut NATIONALLICENCE 700 1- Deering Petra Urologische Klinik und Poliklinik, Technischen Universität München, Ismaningestrasse 22, 81675 München, Germany, DE aut NATIONALLICENCE 700 1- Lehmer Antonie Urologische Klinik und Poliklinik, Technischen Universität München, Ismaningestrasse 22, 81675 München, Germany, DE aut NATIONALLICENCE 700 1- Breul Jürgen Urologische Klinik und Poliklinik, Technischen Universität München, Ismaningestrasse 22, 81675 München, Germany, DE aut NATIONALLICENCE 773 0- World Journal of Surgery Springer-Verlag 24/10(2000-10-01), 1187-1189 0364-2313 24:10<1187 2000 24 268 Metadata rights reserved Springer special CC-BY-NC licence nationallicence BK010053 XK010053 XK010000 NATIONALLICENCE NATIONALLICENCE NL-springer