caa a22 4500 475802802 CHVBK 20180406123742.0 cr unu---uuuuu 170329e20001001xx s 000 0 eng 10.1007/s002680010246 doi (NATIONALLICENCE)springer-10.1007/s002680010246 Helicobacter pylori Infection: Augmentation of Telomerase Activity in Cancer and Noncancerous Tissues [Elektronische Daten] [Hidekazu Kameshima, Atsuhito Yagihashi, Tomomi Yajima, Daisuke Kobayashi, Ryuichi Denno, Koichi Hirata, Naoki Watanabe] Abstract.: Telomerase adds hexameric repeats of 5′-TTAGGG-3′ to the ends of chromosomal DNA (telomere) and has been implicated in cell immortalization and cellular senescence. The aim of this study was to measure quantitatively the telomerase activity and human telomerase RNA component (hTR) content in gastric cancer and to examine the relation between these values and histologic factors including Helicobacter pylori as a risk factor for gastric cancer. Telomerase activity was measured by a modified telomeric repeat amplification protocol in cancerous and noncancerous tissues (intestinal metaplasia, chronic gastritis, normal mucosa) from 27 gastric cancer patients; hTR expression was examined by the quantitative reverse transcriptase-polymerase chain reaction using fluorescent probes. Telomerase activity was higher in cancers (total product generated: 33.7) than in noncancerous tissues. Telomerase activity was higher in intestinal metaplasia (16.7) and chronic gastritis (10.6) than in normal mucosa (3.5). In patients with intestinal-type gastric cancer, telomerase activity was higher in intestinal metaplasia with H. pylori infection than in that without infection. hTR expression was not correlated with telomerase activity. H. pylori infection may influence telomerase activity in cancer and noncancerous tissues. by Société Internationale de Chirurgie, 2000 Kameshima Hidekazu Department of Clinical Laboratory Medicine, Sapporo Medical University School of Medicine, South-1, West-16, Chuo-ku, Sapporo 060-8543, Japan, JP aut Yagihashi Atsuhito Department of Clinical Laboratory Medicine, Sapporo Medical University School of Medicine, South-1, West-16, Chuo-ku, Sapporo 060-8543, Japan, JP aut Yajima Tomomi Department of Clinical Laboratory Medicine, Sapporo Medical University School of Medicine, South-1, West-16, Chuo-ku, Sapporo 060-8543, Japan, JP aut Kobayashi Daisuke Department of Clinical Laboratory Medicine, Sapporo Medical University School of Medicine, South-1, West-16, Chuo-ku, Sapporo 060-8543, Japan, JP aut Denno Ryuichi 1st Department of Surgery, Sapporo Medical University School of Medicine, South-1, West-16, Chuo-ku, Sapporo 060-8543, Japan, JP aut Hirata Koichi 1st Department of Surgery, Sapporo Medical University School of Medicine, South-1, West-16, Chuo-ku, Sapporo 060-8543, Japan, JP aut Watanabe Naoki Department of Clinical Laboratory Medicine, Sapporo Medical University School of Medicine, South-1, West-16, Chuo-ku, Sapporo 060-8543, Japan, JP aut World Journal of Surgery Springer-Verlag 24/10(2000-10-01), 1243-1249 0364-2313 24:10<1243 2000 24 268 https://doi.org/10.1007/s002680010246 text/html Onlinezugriff via DOI 1 research-article jats NATIONALLICENCE 856 40 https://doi.org/10.1007/s002680010246 text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Kameshima Hidekazu Department of Clinical Laboratory Medicine, Sapporo Medical University School of Medicine, South-1, West-16, Chuo-ku, Sapporo 060-8543, Japan, JP aut NATIONALLICENCE 700 1- Yagihashi Atsuhito Department of Clinical Laboratory Medicine, Sapporo Medical University School of Medicine, South-1, West-16, Chuo-ku, Sapporo 060-8543, Japan, JP aut NATIONALLICENCE 700 1- Yajima Tomomi Department of Clinical Laboratory Medicine, Sapporo Medical University School of Medicine, South-1, West-16, Chuo-ku, Sapporo 060-8543, Japan, JP aut NATIONALLICENCE 700 1- Kobayashi Daisuke Department of Clinical Laboratory Medicine, Sapporo Medical University School of Medicine, South-1, West-16, Chuo-ku, Sapporo 060-8543, Japan, JP aut NATIONALLICENCE 700 1- Denno Ryuichi 1st Department of Surgery, Sapporo Medical University School of Medicine, South-1, West-16, Chuo-ku, Sapporo 060-8543, Japan, JP aut NATIONALLICENCE 700 1- Hirata Koichi 1st Department of Surgery, Sapporo Medical University School of Medicine, South-1, West-16, Chuo-ku, Sapporo 060-8543, Japan, JP aut NATIONALLICENCE 700 1- Watanabe Naoki Department of Clinical Laboratory Medicine, Sapporo Medical University School of Medicine, South-1, West-16, Chuo-ku, Sapporo 060-8543, Japan, JP aut NATIONALLICENCE 773 0- World Journal of Surgery Springer-Verlag 24/10(2000-10-01), 1243-1249 0364-2313 24:10<1243 2000 24 268 Metadata rights reserved Springer special CC-BY-NC licence nationallicence BK010053 XK010053 XK010000 NATIONALLICENCE NATIONALLICENCE NL-springer