caa a22 4500 475802837 CHVBK 20180406123742.0 cr unu---uuuuu 170329e20000401xx s 000 0 eng 10.1007/s002689910071 doi (NATIONALLICENCE)springer-10.1007/s002689910071 Free Radical Scavenging Activity of Fullerenol on the Ischemia-reperfusion Intestine in Dogs [Elektronische Daten] [Hong-Shiee Lai, Wei-Jao Chen, Long-Yong Chiang] Abstract.: Fullerenol, a water-soluble C60-fullerene derivative, has been demonstrated to have the capability to scavenge free radicals in vitro and in vivo. The purpose of this study was to investigate whether fullerenol can scavenge the free radicals that are massively induced during ischemia-reperfusion (I/R) injury of the small intestine, either preventively or therapeutically. Clamping the superior mesenteric artery and vein for 60 minutes to induce I/R injury was performed on male mongrel dogs. Thirty dogs were divided into three groups (10 in each): The control (C) group received no medication; the preventive (P) group received fullerenol (1 mg/kg) intravenously 30 minutes before ischemia; the therapeutic (T) group received the same dose of fullerenol immediately after reperfusion. This study was an experimental randomized trial. Intestinal segments were obtained 10, 20, 30, and 60 minutes after reperfusion; and blood samples and specimens of major organs were taken 60 minutes after reperfusion. Concentrations of lipid peroxidation products, including conjugated diene (CD) and malondialdehyde (MDA), and the level of glutathione (GSH) in intestinal tissue were determined. Serum indicators of liver and renal function were measured. Histologic examination of the small intestine and major organs were also performed. A significant increase in intestinal MDA and CD contents was detected at 30 and 60 minutes after reperfusion. The tissue GSH content, in contrast, was decreased 60 minutes after reperfusion. Administration of fullerenol diminished these changes both preventively and therapeutically. Liver and renal functions were within normal limits in all groups. Moreover no obvious histopathologic additional damage could be found in either the P or the T group. It is suggested that fullerenol can be considered a powerful scavenger for the free radicals induced by I/R injury of the small intestine. Société Internationale de Chirurgie, 2000 Lai Hong-Shiee Department of Surgery, National Taiwan University Hospital, No. 7 Chung-Shan South Road, Taipei, Taiwan, TW aut Chen Wei-Jao Department of Surgery, National Taiwan University Hospital, No. 7 Chung-Shan South Road, Taipei, Taiwan, TW aut Chiang Long-Yong Department of Surgery, National Taiwan University Hospital, No. 7 Chung-Shan South Road, Taipei, Taiwan, TW aut World Journal of Surgery Springer-Verlag 24/4(2000-04-01), 450-454 0364-2313 24:4<450 2000 24 268 https://doi.org/10.1007/s002689910071 text/html Onlinezugriff via DOI 1 research-article jats NATIONALLICENCE 856 40 https://doi.org/10.1007/s002689910071 text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Lai Hong-Shiee Department of Surgery, National Taiwan University Hospital, No. 7 Chung-Shan South Road, Taipei, Taiwan, TW aut NATIONALLICENCE 700 1- Chen Wei-Jao Department of Surgery, National Taiwan University Hospital, No. 7 Chung-Shan South Road, Taipei, Taiwan, TW aut NATIONALLICENCE 700 1- Chiang Long-Yong Department of Surgery, National Taiwan University Hospital, No. 7 Chung-Shan South Road, Taipei, Taiwan, TW aut NATIONALLICENCE 773 0- World Journal of Surgery Springer-Verlag 24/4(2000-04-01), 450-454 0364-2313 24:4<450 2000 24 268 Metadata rights reserved Springer special CC-BY-NC licence nationallicence BK010053 XK010053 XK010000 NATIONALLICENCE NATIONALLICENCE NL-springer