caa a22 4500 475803345 CHVBK 20180406123743.0 cr unu---uuuuu 170329e20000501xx s 000 0 eng 10.1007/s002689910080 doi (NATIONALLICENCE)springer-10.1007/s002689910080 Release of Endotoxin-binding Proteins during Major Elective Surgery: Role of Soluble CD14 in Phagocytic Activation [Elektronische Daten] [Naoki Hiki, Dieter Berger, Yoshikazu Mimura, Julia Frick, Mieke A. Dentener, Wim A. Buurman, Manuela Seidelmann, Michio Kaminishi, Hans G. Beger] Abstract.: Our previous study demonstrated that soluble CD14 (sCD14) modulates the biologic activity of circulating endotoxin, which appears after surgery. In this study, we examined the behavior of endotoxin-binding proteins, such as sCD14, lipopolysaccharide-binding protein (LBP), and bactericidal/permeability-increasing protein (BPI), in patients' plasma after major abdominal surgery and the phagocytic secretion of sCD14 from peripheral blood mononuclear cells (PBMCs) throughout the observation period. In a prospective study, 15 patients undergoing major abdominal surgery (gastrectomy, n= 3; pancreatectomy, n= 10; colectomy, n= 2) were involved in this study. The endotoxin-binding proteins were perioperatively (preoperatively; postoperative hour 6; days 1, 2, 3, 4, 5, 7, and 10) measured by an enzyme-linked immunosorbent assay (ELISA). To exclude the hemodilution effect of samples, each parameter was corrected by dividing the respective value by the albumin concentration. The phagocytic activity at each time point was tested as an ex vivo sCD14 secretion from PBMCs in the presence and absence of exogenously added endotoxin, Escherichia coli 055B5 (1 ng/ml). Significant endotoxemia (0.35 ± 0.13 EU/ml; p < 0.05) was observed 6 hours after the beginning of surgery. The sCD14/albumin value rapidly increased at 6 hours after surgery, peaked on day 1, and sequentially declined, whereas the BPI/albumin and LBP/albumin ratios increased more gradually and peaked on day 2. The secretion of sCD14 from 2 × 106 PBMCs was significantly enhanced from 6 hours after operation. The increased plasma level of sCD14 may be explained by the parallel-enhanced sCD14 PBMC production. Activated secretion of these endotoxin-binding proteins may play a role in regulating the biologic activity of circulating endotoxin. 2000 by the Société Internationale de Chir, 2000 Hiki Naoki Department of Surgery, University of Tokyo, 3-28-6 Mejirodai, Bunkyo-ku, Tokyo 112-8688, Japan, JP aut Berger Dieter Department of General Surgery, University of Ulm, Steinhoevelstr. 9, 89075 Ulm, Germany, DE aut Mimura Yoshikazu Department of Surgery, University of Tokyo, 3-28-6 Mejirodai, Bunkyo-ku, Tokyo 112-8688, Japan, JP aut Frick Julia Department of General Surgery, University of Ulm, Steinhoevelstr. 9, 89075 Ulm, Germany, DE aut Dentener Mieke A. Department of Pulmonology, University of Maastricht, P.O. Box 616, 6200 AZ Maastricht, The Netherlands, NL aut Buurman Wim A. Department of Surgery, University of Maastricht, P.O. Box 616, 6200 AZ Maastricht, The Netherlands, NL aut Seidelmann Manuela Department of General Surgery, University of Ulm, Steinhoevelstr. 9, 89075 Ulm, Germany, DE aut Kaminishi Michio Department of Surgery, University of Tokyo, 3-28-6 Mejirodai, Bunkyo-ku, Tokyo 112-8688, Japan, JP aut Beger Hans G. Department of General Surgery, University of Ulm, Steinhoevelstr. 9, 89075 Ulm, Germany, DE aut World Journal of Surgery Springer-Verlag 24/5(2000-05-01), 499-506 0364-2313 24:5<499 2000 24 268 https://doi.org/10.1007/s002689910080 text/html Onlinezugriff via DOI 1 research-article jats NATIONALLICENCE 856 40 https://doi.org/10.1007/s002689910080 text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Hiki Naoki Department of Surgery, University of Tokyo, 3-28-6 Mejirodai, Bunkyo-ku, Tokyo 112-8688, Japan, JP aut NATIONALLICENCE 700 1- Berger Dieter Department of General Surgery, University of Ulm, Steinhoevelstr. 9, 89075 Ulm, Germany, DE aut NATIONALLICENCE 700 1- Mimura Yoshikazu Department of Surgery, University of Tokyo, 3-28-6 Mejirodai, Bunkyo-ku, Tokyo 112-8688, Japan, JP aut NATIONALLICENCE 700 1- Frick Julia Department of General Surgery, University of Ulm, Steinhoevelstr. 9, 89075 Ulm, Germany, DE aut NATIONALLICENCE 700 1- Dentener Mieke A. Department of Pulmonology, University of Maastricht, P.O. Box 616, 6200 AZ Maastricht, The Netherlands, NL aut NATIONALLICENCE 700 1- Buurman Wim A. Department of Surgery, University of Maastricht, P.O. Box 616, 6200 AZ Maastricht, The Netherlands, NL aut NATIONALLICENCE 700 1- Seidelmann Manuela Department of General Surgery, University of Ulm, Steinhoevelstr. 9, 89075 Ulm, Germany, DE aut NATIONALLICENCE 700 1- Kaminishi Michio Department of Surgery, University of Tokyo, 3-28-6 Mejirodai, Bunkyo-ku, Tokyo 112-8688, Japan, JP aut NATIONALLICENCE 700 1- Beger Hans G. Department of General Surgery, University of Ulm, Steinhoevelstr. 9, 89075 Ulm, Germany, DE aut NATIONALLICENCE 773 0- World Journal of Surgery Springer-Verlag 24/5(2000-05-01), 499-506 0364-2313 24:5<499 2000 24 268 Metadata rights reserved Springer special CC-BY-NC licence nationallicence BK010053 XK010053 XK010000 NATIONALLICENCE NATIONALLICENCE NL-springer