caa a22 4500 475803647 CHVBK 20180406123744.0 cr unu---uuuuu 170329e20001101xx s 000 0 eng 10.1007/s002680010225 doi (NATIONALLICENCE)springer-10.1007/s002680010225 Parathyroid Xenotransplantation without Immunosuppression in Experimental Hypoparathyroidism: Long-term In Vivo Function following Microencapsulation with a Clinically Suitable Alginate [Elektronische Daten] [Christian Hasse, Thomas Bohrer, Peter Barth, Benno Stinner, Richard Cohen, Hubert Cramer, Ulrich Zimmermann, Matthias Rothmund] Permanent hypoparthyroidism is one of the most difficult of all endocrine disorders to treat medically. Because this deficiency syndrome rarely is a life-threatening condition, systemic immunosuppression for recipients of transgenic transplants is not justified. An alternative would be protecting the tissue to be transplanted from the immunologic response (immunoisolation) by coating it with a semipermeable membrane- microencapsulation. Unfortunately, prior to the first clinical use, further analysis of the coating substance (alginate) demonstrated that it has potential cancerogenic properties. Using a purified amitogenic alginate suitable for clinical use, we accomplished allotransplantation in a long-term animal model and reported the first clinical cases without postoperative immunosuppression recently. In view of the potential clinical use, we investigated the ability of the microencapsulation technology with the novel amitogenic alginate in experimental hypoparathyroidism (80 parathyroidectomized rats) to enable transgenic transplantation across the highest immunologic barrier (xenotransplantation: human to rat) to ensure intact transplant function without immunosuppression. In a controlled, long-term animal study, the effect of microencapsulation on xenotransplanted human parathyroid tissue was evaluated over a period of 30 weeks (microencapsulated and naked hyperplastic parathyroid tissue, respectively). Functionally, human parathyroid tissue was able to replace that of rats. More than 6 months after xenotransplantation 32 of 40 animals that had received microencapsulated transplants were normocalcemic. In contrast, serum calcium concentrations dropped to postparathyroidectomy levels within 3 weeks in the animals that had received naked tissue only. Correspondingly, normocalcemic animals showed vital parathyroid tissue inside the microcapsules, which were surrounded by a small rim of fibroblasts. When combining microencapsulation with an improved tissue culture method, xenotransplantation of human parathyroid tissue and maintenance of its physiologic function is reproducibly achieved over the highest transplantation barrier. Using the amitogenic alginate may be a crucial step toward the first clinical use of this technique for parathyroid xenotransplantation without immunosuppression. Société Internationale de Chirurgie, 2000 Hasse Christian Department of Surgery, Philipps-University of Marburg, Baldingerstrasse, 35043 Marburg, Germany, DE aut Bohrer Thomas Department of Surgery, Philipps-University of Marburg, Baldingerstrasse, 35043 Marburg, Germany, DE aut Barth Peter Institute of Pathology, Philipps-University of Marburg, Baldingerstrasse, 35043 Marburg, Germany, DE aut Stinner Benno Department of Surgery, Philipps-University of Marburg, Baldingerstrasse, 35043 Marburg, Germany, DE aut Cohen Richard St. Mark's Hospital, North West London NHS, Watford Road, Harrow HA1 3UJ, UK, GB aut Cramer Hubert Institute of Biotechnology, Julius-Maximilians University of Würzburg, Am Hubland, 97074 Würzburg, Germany, DE aut Zimmermann Ulrich Institute of Biotechnology, Julius-Maximilians University of Würzburg, Am Hubland, 97074 Würzburg, Germany, DE aut Rothmund Matthias Department of Surgery, Philipps-University of Marburg, Baldingerstrasse, 35043 Marburg, Germany, DE aut World Journal of Surgery Springer-Verlag 24/11(2000-11-01), 1361-1366 0364-2313 24:11<1361 2000 24 268 https://doi.org/10.1007/s002680010225 text/html Onlinezugriff via DOI 1 research-article jats NATIONALLICENCE 856 40 https://doi.org/10.1007/s002680010225 text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Hasse Christian Department of Surgery, Philipps-University of Marburg, Baldingerstrasse, 35043 Marburg, Germany, DE aut NATIONALLICENCE 700 1- Bohrer Thomas Department of Surgery, Philipps-University of Marburg, Baldingerstrasse, 35043 Marburg, Germany, DE aut NATIONALLICENCE 700 1- Barth Peter Institute of Pathology, Philipps-University of Marburg, Baldingerstrasse, 35043 Marburg, Germany, DE aut NATIONALLICENCE 700 1- Stinner Benno Department of Surgery, Philipps-University of Marburg, Baldingerstrasse, 35043 Marburg, Germany, DE aut NATIONALLICENCE 700 1- Cohen Richard St. Mark's Hospital, North West London NHS, Watford Road, Harrow HA1 3UJ, UK, GB aut NATIONALLICENCE 700 1- Cramer Hubert Institute of Biotechnology, Julius-Maximilians University of Würzburg, Am Hubland, 97074 Würzburg, Germany, DE aut NATIONALLICENCE 700 1- Zimmermann Ulrich Institute of Biotechnology, Julius-Maximilians University of Würzburg, Am Hubland, 97074 Würzburg, Germany, DE aut NATIONALLICENCE 700 1- Rothmund Matthias Department of Surgery, Philipps-University of Marburg, Baldingerstrasse, 35043 Marburg, Germany, DE aut NATIONALLICENCE 773 0- World Journal of Surgery Springer-Verlag 24/11(2000-11-01), 1361-1366 0364-2313 24:11<1361 2000 24 268 Metadata rights reserved Springer special CC-BY-NC licence nationallicence BK010053 XK010053 XK010000 NATIONALLICENCE NATIONALLICENCE NL-springer