caa a22 4500 475806247 CHVBK 20180406123747.0 cr unu---uuuuu 170329e20000301xx s 000 0 eng 10.1007/BF00985460 doi (NATIONALLICENCE)springer-10.1007/BF00985460 Inter- and intraspecific genetic variation in Hippophae (Elaeagnaceae) investigated by RAPD markers [Elektronische Daten] [I. Bartish, N. Jeppsson, G. Bartish, R. Lu, H. Nybom] Genetic diversity has been investigated by the application of molecular markers in, for the first time, all the taxa recognised in recent treatises of the genusHippophae. RAPD (random amplified polymorphic DNA) analyses were conducted with 9 decamer primers, which together yielded 219 polymorphic markers. We found 16 fixed RAPD markers, i.e. markers that either occurred in all plants of a population or were absent from all plants. Several of these markers were useful for analysis of interspecific relationships, whereas others can be considered as taxon-specific markers. Clustering of taxa and populations in our neighbour-joining based dendrogram was in good agreement with some recently suggested taxonomic treatises ofHippophae. Amount and distribution of genetic variability varied considerably between species. Partitioning of molecular variance withinH. rhamnoides supported earlier findings that a considerable part of the total variance resides among subspecies (59.6%) Within-population variability also differed considerably. Percentage polymorphic RAPD loci and Lynch and Milligan within-population gene diversity estimates showed relatively high values for some species close to the geographic centre of origin in Central Asia, e.g.H. tibetana and the putatively hybridogenousH. goniocarpa. Spatial autocorrelation analyses performed on 12 populations ofH. rhamnoides revealed positive autocorrelation of allele frequencies when geographic distances ranged from 0 to 700 km, and no or negative autocorrelation at higher distances. At distances between 700 and 1900 km, we observed deviations from the expected values with strongly negative autocorrelation of allele frequencies. A corresponding relationship between geographic and genetic distances could not be found when the analysis instead was based on one population from each of 8 species. Springer-Verlag, 2000 Elaeagnaceae nationallicence Hippophae nationallicence sea buckthorn nationallicence Systematics nationallicence taxonomy nationallicence genetic variation nationallicence RAPD nationallicence Bartish I. Institute of Botany, Academy of Sciences, C2-25243, Pruhonice 1, Czech Republic aut Jeppsson N. Balsgård-Department of Horticultural Plant Breeding, Swedish University of Agricultural Sciences, Fjälkestadsvägen 123-1, SE-291 94, Kristianstad, Sweden aut Bartish G. Balsgård-Department of Horticultural Plant Breeding, Swedish University of Agricultural Sciences, Fjälkestadsvägen 123-1, SE-291 94, Kristianstad, Sweden aut Lu R. Chengdu Institute of Biology, Chinese Academy of Sciences, P.O. Box 416, 610041, Chengdu, Sichuan, China aut Nybom H. Balsgård-Department of Horticultural Plant Breeding, Swedish University of Agricultural Sciences, Fjälkestadsvägen 123-1, SE-291 94, Kristianstad, Sweden aut Plant Systematics and Evolution Springer-Verlag 225/1-4(2000-03-01), 85-101 0378-2697 225:1-4<85 2000 225 606 https://doi.org/10.1007/BF00985460 text/html Onlinezugriff via DOI 1 research-article jats NATIONALLICENCE 856 40 https://doi.org/10.1007/BF00985460 text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Bartish I. Institute of Botany, Academy of Sciences, C2-25243, Pruhonice 1, Czech Republic aut NATIONALLICENCE 700 1- Jeppsson N. Balsgård-Department of Horticultural Plant Breeding, Swedish University of Agricultural Sciences, Fjälkestadsvägen 123-1, SE-291 94, Kristianstad, Sweden aut NATIONALLICENCE 700 1- Bartish G. Balsgård-Department of Horticultural Plant Breeding, Swedish University of Agricultural Sciences, Fjälkestadsvägen 123-1, SE-291 94, Kristianstad, Sweden aut NATIONALLICENCE 700 1- Lu R. Chengdu Institute of Biology, Chinese Academy of Sciences, P.O. Box 416, 610041, Chengdu, Sichuan, China aut NATIONALLICENCE 700 1- Nybom H. Balsgård-Department of Horticultural Plant Breeding, Swedish University of Agricultural Sciences, Fjälkestadsvägen 123-1, SE-291 94, Kristianstad, Sweden aut NATIONALLICENCE 773 0- Plant Systematics and Evolution Springer-Verlag 225/1-4(2000-03-01), 85-101 0378-2697 225:1-4<85 2000 225 606 Metadata rights reserved Springer special CC-BY-NC licence nationallicence BK010053 XK010053 XK010000 NATIONALLICENCE NATIONALLICENCE NL-springer