caa a22 4500 47580631X CHVBK 20180406123747.0 cr unu---uuuuu 170329e20000301xx s 000 0 eng 10.1007/BF00985457 doi (NATIONALLICENCE)springer-10.1007/BF00985457 Heterogeneity of the internal transcribed spacer 1 (ITS1) in Tulipa (Liliaceae) [Elektronische Daten] [G. Booy, J. Van der Schoot, B. Vosman] Heterogeneity of the internal transcribed spacer ITS1 of the rDNA within individuals ofTulipa gesneriana L.,T. kaufmanniana Regel, and their interspecific hybrids was analyzed by PCRRFLP, using the polymorphic restriction enzymesRsaI andHinfI, and by nucleotide sequence analysis. In most cases, the sum of the sizes of the restriction fragments was higher than the entire length of the undigested ITS fragment, indicating heterogeneity at the restriction sites within an individual. Differences in band intensities within the restriction patterns indicate the occurrence of variation in copy number of these different ITS1 variants within individuals. Automated sequencing without a visual inspection often failed to detect existing heterogeneity within sequences, resulting in a discrepancy between the sequencing and restriction analysis results. By visual interpretation of the sequences, the restriction patterns could mostly be predicted well. Fluorescence in situ hybridization (FISH) experiments in fourTulipa species revealed the occurrence of several rDNA spots. The number of rDNA loci varied from seven inT. gesneriana ‘Christmas Marvel' to ten inT. australis Link. This might explain the occurrence of heterogeneity in ITS sequences inTulipa, as homogenization of variants has to take place over different loci. Springer-Verlag, 2000 Liliaceae nationallicence Tulipa nationallicence inheritance nationallicence internal transcribed spacer (ITS) nationallicence heterogeneity nationallicence restriction patterns nationallicence sequencing nationallicence chromosomal localization nationallicence Booy G. Plant Research International, P.O. Box 16, 6700 AA, Wageningen, The Netherlands aut Van der Schoot J. Plant Research International, P.O. Box 16, 6700 AA, Wageningen, The Netherlands aut Vosman B. Plant Research International, P.O. Box 16, 6700 AA, Wageningen, The Netherlands aut Plant Systematics and Evolution Springer-Verlag 225/1-4(2000-03-01), 29-41 0378-2697 225:1-4<29 2000 225 606 https://doi.org/10.1007/BF00985457 text/html Onlinezugriff via DOI 1 research-article jats NATIONALLICENCE 856 40 https://doi.org/10.1007/BF00985457 text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Booy G. Plant Research International, P.O. Box 16, 6700 AA, Wageningen, The Netherlands aut NATIONALLICENCE 700 1- Van der Schoot J. Plant Research International, P.O. Box 16, 6700 AA, Wageningen, The Netherlands aut NATIONALLICENCE 700 1- Vosman B. Plant Research International, P.O. Box 16, 6700 AA, Wageningen, The Netherlands aut NATIONALLICENCE 773 0- Plant Systematics and Evolution Springer-Verlag 225/1-4(2000-03-01), 29-41 0378-2697 225:1-4<29 2000 225 606 Metadata rights reserved Springer special CC-BY-NC licence nationallicence BK010053 XK010053 XK010000 NATIONALLICENCE NATIONALLICENCE NL-springer