caa a22 4500 475806328 CHVBK 20180406123747.0 cr unu---uuuuu 170329e20000301xx s 000 0 eng 10.1007/BF00985455 doi (NATIONALLICENCE)springer-10.1007/BF00985455 Nuclear ribosomal ITS sequences and phylogeny in East Asian Aconitum subgenus Aconitum (Ranunculaceae), with special reference to extensive polymorphism in individual plants [Elektronische Daten] [Y. Kita, M. Ito] Internal transcribed spacer (ITS) sequences of nuclear ribosomal DNA (nrDNA) were used to examine the phylogeny of East Asian aconites. Individual aconites were discovered to contain as many as eight different ITS sequences after cloning and PCR-SSCP (single-stranded conformational polymorphisms) analysis. We identified eight putative ITS pseudogenes from four taxa with low predicted secondary structure stability and high substitution rates. Maximum likelihood (ML) and neighbor-joining (NJ) methods were used for phylogenetic reconstruction. The ITS trees agree with the previous chloroplast DNA (cpDNA) tree for the vast majority of the taxa. We found two East Asian clades in the ITS trees: 1) a clade with the Chinese diploid,Aconitum volubile and East Asian tetraploids, and 2) a clade of East Asian diploids and Siberian tetraploids. In the former clade, most tetraploid taxa appear to be polyphyletic; sequences from individual plants did not correspond to recognized taxonomic units. This indicates a recent divergence of the East Asian tetraploids. Springer-Verlag, 2000 Ranunculaceae nationallicence Aconitum nationallicence ITS nationallicence polymorphism nationallicence pseudogenes nationallicence phylogeny nationallicence Kita Y. Department of Biological Sciences, Graduate School of Science, University of Tokyo, 7-3-1 Hongo, Bunkyo-ku, 113-0033, Tokyo, Japan aut Ito M. Department of Systems Sciences, University of Tokyo, 153-8902, Komaba, Tokyo, Japan aut Plant Systematics and Evolution Springer-Verlag 225/1-4(2000-03-01), 1-13 0378-2697 225:1-4<1 2000 225 606 https://doi.org/10.1007/BF00985455 text/html Onlinezugriff via DOI 1 research-article jats NATIONALLICENCE 856 40 https://doi.org/10.1007/BF00985455 text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Kita Y. Department of Biological Sciences, Graduate School of Science, University of Tokyo, 7-3-1 Hongo, Bunkyo-ku, 113-0033, Tokyo, Japan aut NATIONALLICENCE 700 1- Ito M. Department of Systems Sciences, University of Tokyo, 153-8902, Komaba, Tokyo, Japan aut NATIONALLICENCE 773 0- Plant Systematics and Evolution Springer-Verlag 225/1-4(2000-03-01), 1-13 0378-2697 225:1-4<1 2000 225 606 Metadata rights reserved Springer special CC-BY-NC licence nationallicence BK010053 XK010053 XK010000 NATIONALLICENCE NATIONALLICENCE NL-springer