caa a22 4500 475817354 CHVBK 20180406123812.0 cr unu---uuuuu 170329e20000601xx s 000 0 eng 10.1007/PL00009836 doi (NATIONALLICENCE)springer-10.1007/PL00009836 Isolation and characterisation of two pollen-specific LIM domain protein cDNAs from Nicotiana tabacum [Elektronische Daten] [J. Sweetman, C. Spurr, Å. Eliasson, N. Gass, A. Steinmetz, D. Twell] Abstract : A PCR-generated DNA probe covering the region corresponding to the two contiguous LIM domain sequences from the sunflower PLIM-1 cDNA was used to screen a cDNA library prepared from poly-A+ RNA isolated from mature Nicotiana tabacum pollen. Two clones were isolated and found to have unique but closely related nucleotide sequences NTPLIM1a and NTPLIM1b. Homology searches revealed the presence of two LIM domain repeats in each clone. Detailed spatial and temporal northern blot analysis showed that the NTPLIM transcripts were present specifically in mature and germinating pollen and were detectable immediately before the asymmetrical division pollen mitosis I, reaching a maximal level in mature pollen. Southern blot analysis revealed the NTPLIM1a and NTPLIM1b genes to be single-copy and members of a small gene family in the N. tabacum genome. Analysis of a genomic clone encoding NTPLIM1a revealed that the gene is split by four introns ranging from 79 to 922 bp in length. The high levels of developmentally regulated expression specifically in pollen suggests an important role for these proteins in pollen maturation and/or function. Springer-Verlag Berlin Heidelberg, 2000 Key words LIM cDNAs nationallicence Pollen-specific nationallicence Zinc finger nationallicence Nicotiana tabacum nationallicence Sweetman J. Department of Biology, University of Leicester, University Road, Leicester LE1 7RH, UK e-mail: twe@le.ac.uk Tel.: +44-116-252 2281, Fax: +44-116-252 2791, GB aut Spurr C. Department of Biology, University of Leicester, University Road, Leicester LE1 7RH, UK e-mail: twe@le.ac.uk Tel.: +44-116-252 2281, Fax: +44-116-252 2791, GB aut Eliasson Å. IBMP-CNRS, 12 rue du Général Zimmer, Strasbourg, France, FR aut Gass N. IBMP-CNRS, 12 rue du Général Zimmer, Strasbourg, France, FR aut Steinmetz A. IBMP-CNRS, 12 rue du Général Zimmer, Strasbourg, France, FR aut Twell D. Department of Biology, University of Leicester, University Road, Leicester LE1 7RH, UK e-mail: twe@le.ac.uk Tel.: +44-116-252 2281, Fax: +44-116-252 2791, GB aut https://doi.org/10.1007/PL00009836 text/html Onlinezugriff via DOI 1 research-article jats NATIONALLICENCE 856 40 https://doi.org/10.1007/PL00009836 text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Sweetman J. Department of Biology, University of Leicester, University Road, Leicester LE1 7RH, UK e-mail: twe@le.ac.uk Tel.: +44-116-252 2281, Fax: +44-116-252 2791, GB aut NATIONALLICENCE 700 1- Spurr C. Department of Biology, University of Leicester, University Road, Leicester LE1 7RH, UK e-mail: twe@le.ac.uk Tel.: +44-116-252 2281, Fax: +44-116-252 2791, GB aut NATIONALLICENCE 700 1- Eliasson Å. IBMP-CNRS, 12 rue du Général Zimmer, Strasbourg, France, FR aut NATIONALLICENCE 700 1- Gass N. IBMP-CNRS, 12 rue du Général Zimmer, Strasbourg, France, FR aut NATIONALLICENCE 700 1- Steinmetz A. IBMP-CNRS, 12 rue du Général Zimmer, Strasbourg, France, FR aut NATIONALLICENCE 700 1- Twell D. Department of Biology, University of Leicester, University Road, Leicester LE1 7RH, UK e-mail: twe@le.ac.uk Tel.: +44-116-252 2281, Fax: +44-116-252 2791, GB aut Metadata rights reserved Springer special CC-BY-NC licence nationallicence BK010053 XK010053 XK010000 NATIONALLICENCE NATIONALLICENCE NL-springer