caa a22 4500 475817362 CHVBK 20180406123812.0 cr unu---uuuuu 170329e20000601xx s 000 0 eng 10.1007/s004970000022 doi (NATIONALLICENCE)springer-10.1007/s004970000022 Regulation of LAT52 promoter activity during pollen tube growth through the pistil of Nicotiana alata [Elektronische Daten] [P. D. Gerola, C. A. Mol, E. Newbigin, W. M. Lush] Abstract : The pollen-specific promoter of the LAT52 gene is known to direct expression of marker proteins during the last stages of pollen maturation and in very early pollen tube growth.We have examined the expression of LAT52-GUS during later stages of pollen tube growth in style and ovary of the relatively long-styled species Nicotiana alata. GUS activity was detected histochemically and found to be present in germinating pollen grains of N. alata and in tubes growing through the upper part of the style. No GUS activity was detected in 99% of the pollen tubes growing through the lower part of the style, but activity was present in tubes within the ovary. This finding indicates that the LAT52 promoter is regulated in growing pollen tubes, and is most active during the earliest and latest stages of pollen tube growth. GUS activity was also detected in some ovules, where it presumably marked the release of pollen tube cytoplasm into the ovule. The distribution of ovules with GUS activity within the ovary is not consistent with high-precision pollen tube guidance to the ovule. Springer-Verlag Berlin Heidelberg, 2000 Key words LAT52 nationallicence Pollen nationallicence Ovary nationallicence Reporter gene nationallicence Transcription nationallicence Nicotiana alata nationallicence Gerola P. D. Department of Structural and Functional Biology, University of Insubria, via Henry Dunant 3, Varese, Italy E-mail: paolo.gerola&unimi.it; Fax: +39-332-421331, IT aut Mol C. A. Laboratorium voor Plantenveredeling, Landbouwuniversiteit, Wageningen, The Netherlands, NL aut Newbigin E. Plant Cell Biology Research Centre, School of Botany, University of Melbourne, Parkville, Victoria 3052, Australia, AU aut Lush W. M. Plant Cell Biology Research Centre, School of Botany, University of Melbourne, Parkville, Victoria 3052, Australia, AU aut https://doi.org/10.1007/s004970000022 text/html Onlinezugriff via DOI 1 research-article jats NATIONALLICENCE 856 40 https://doi.org/10.1007/s004970000022 text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Gerola P. D. Department of Structural and Functional Biology, University of Insubria, via Henry Dunant 3, Varese, Italy E-mail: paolo.gerola&unimi.it; Fax: +39-332-421331, IT aut NATIONALLICENCE 700 1- Mol C. A. Laboratorium voor Plantenveredeling, Landbouwuniversiteit, Wageningen, The Netherlands, NL aut NATIONALLICENCE 700 1- Newbigin E. Plant Cell Biology Research Centre, School of Botany, University of Melbourne, Parkville, Victoria 3052, Australia, AU aut NATIONALLICENCE 700 1- Lush W. M. Plant Cell Biology Research Centre, School of Botany, University of Melbourne, Parkville, Victoria 3052, Australia, AU aut Metadata rights reserved Springer special CC-BY-NC licence nationallicence BK010053 XK010053 XK010000 NATIONALLICENCE NATIONALLICENCE NL-springer