caa a22 4500 475817370 CHVBK 20180406123812.0 cr unu---uuuuu 170329e20000601xx s 000 0 eng 10.1007/s004970000027 doi (NATIONALLICENCE)springer-10.1007/s004970000027 Genomic factors responsible for abnormal morphology of pollen tubes in the interspecific cross Nicotiana tabacum × N. rustica [Elektronische Daten] [T. Kuboyama, G. Takeda] Abstract : Nicotiana tabacum shows unilateral pollen-pistil incongruity with N. rustica. If N. tabacum is pollinated with N. rustica, growth of the pollen tube is arrested in the middle of the style, and abundant callose deposition, tube swelling and tube winding are observed. An attempt was made to clarify the genomic factors responsible for this pollen-pistil incongruity. N. tabacum was pollinated with N. paniculata or N. undulata, progenitors of amphidiploid N. rustica. When pollinated with N. undulata, growth of the pollen tube was arrested in the middle of the style and showed abnormal morphology similar to that with N. rustica, but when pollinated with N. paniculata the pollen tube reached near the base of the style and was almost normal in appearance. These observations suggest that the factors responsible for the pollen tube abnormality of N. rustica are derived from the N. undulata genome.We also used N. sylvestris, N. glutinosa and N. otophora as pistilar parents and N. rustica or its progenitors as pollen parents to examine the genomic factors of the pistilate parents. The pollen tube features of these three species in the pistils of N. sylvestris were similar to those in the pistil of N. tabacum. Springer-Verlag Berlin Heidelberg, 2000 Key words Unilateral incongruity nationallicence Interspecific cross nationallicence Gemome nationallicence Solanaceae nationallicence Tobacco nationallicence Kuboyama T. Department of Agricultural and Environmental Biology, Graduate School of Agricultural and Life Sciences, The University of Tokyo, 1-1-1 Yayoi, Bunkyo-ku, Tokyo 113, Japan e-mail: akuboya@mail.ecc.u-tokyo.ac.jp Fax +81-3-5841-5070, Tel. +81-3-5841-28131, JP aut Takeda G. Laboratory of Plant Breeding, Faculty of Agriculture, Tamagawa University, Machida, Tokyo 194, Japan, JP aut https://doi.org/10.1007/s004970000027 text/html Onlinezugriff via DOI 1 research-article jats NATIONALLICENCE 856 40 https://doi.org/10.1007/s004970000027 text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Kuboyama T. Department of Agricultural and Environmental Biology, Graduate School of Agricultural and Life Sciences, The University of Tokyo, 1-1-1 Yayoi, Bunkyo-ku, Tokyo 113, Japan e-mail: akuboya@mail.ecc.u-tokyo.ac.jp Fax +81-3-5841-5070, Tel. +81-3-5841-28131, JP aut NATIONALLICENCE 700 1- Takeda G. Laboratory of Plant Breeding, Faculty of Agriculture, Tamagawa University, Machida, Tokyo 194, Japan, JP aut Metadata rights reserved Springer special CC-BY-NC licence nationallicence BK010053 XK010053 XK010000 NATIONALLICENCE NATIONALLICENCE NL-springer