caa a22 4500 475817435 CHVBK 20180406123812.0 cr unu---uuuuu 170329e20000101xx s 000 0 eng 10.1007/s004970050010 doi (NATIONALLICENCE)springer-10.1007/s004970050010 Molecular characterization of an anther-specific gene from tobacco shows sequence similarity to a tapetum-specific gene from tomato [Elektronische Daten] [S. I. Fuerstenberg, P. B. Bucciaglia, A. G. Smith] Abstract : We have cloned and determined the DNA sequence of the cDNA of ntGRP15. The cDNA ntGRP15 represents an anther-specific, developmentally regulated gene from Nicotiana tabacum that encodes a glycine-rich protein. Northern analysis shows that the gene is specifically expressed in anthers and is stringently regulated during anther development. It appears only in anthers at the meiosis to free microspore stages of development. The encoded protein is small (12.2 kDa), has a 31% glycine content and contains a putative signal sequence. By both nucleotide and amino acid sequence alignment, the gene shows high sequence similarity to a gene previously isolated from Lycopersicon esculentum, namely, TomA92b9. High glycine content, presence of a signal sequence and similarity to the tomato TomA92b9 gene suggests the protein functions as a structural cell wall protein, possibly involved in pollen exine formation. Springer-Verlag Berlin Heidelberg, 2000 Key words Glycine-rich nationallicence Tobacco nationallicence Tomato Pollen development nationallicence Signal sequence nationallicence Fuerstenberg S. I. Biology Department, University of Michigan, 830 N. University Ave. Ann Arbor, MI 48109, USA e-mail: sifuerst@umich.edu Fax: +1-734-647-0884, US aut Bucciaglia P. B. Department of Horticultural Science, University of Minnesota, 305 Alderman Hall, St. Paul, MN 55108, USA, US aut Smith A. G. Department of Horticultural Science, University of Minnesota, 305 Alderman Hall, St. Paul, MN 55108, USA, US aut https://doi.org/10.1007/s004970050010 text/html Onlinezugriff via DOI 1 research-article jats NATIONALLICENCE 856 40 https://doi.org/10.1007/s004970050010 text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Fuerstenberg S. I. Biology Department, University of Michigan, 830 N. University Ave. Ann Arbor, MI 48109, USA e-mail: sifuerst@umich.edu Fax: +1-734-647-0884, US aut NATIONALLICENCE 700 1- Bucciaglia P. B. Department of Horticultural Science, University of Minnesota, 305 Alderman Hall, St. Paul, MN 55108, USA, US aut NATIONALLICENCE 700 1- Smith A. G. Department of Horticultural Science, University of Minnesota, 305 Alderman Hall, St. Paul, MN 55108, USA, US aut Metadata rights reserved Springer special CC-BY-NC licence nationallicence BK010053 XK010053 XK010000 NATIONALLICENCE NATIONALLICENCE NL-springer