caa a22 4500 475817486 CHVBK 20180406123812.0 cr unu---uuuuu 170329e20000101xx s 000 0 eng 10.1007/s004970050005 doi (NATIONALLICENCE)springer-10.1007/s004970050005 Using S -locus deletions to evaluate self-incompatibility candidate genes: an example for a novel anther-expressed gene [Elektronische Daten] [A. L. Casselman, M. E. Nasrallah, J. B. Nasrallah] Abstract : A novel gene, designated SPA, was isolated using a differential display strategy designed to target the Brassica self-incompatibility (S) locus for anther-expressed sequences. SPA is genetically and physically linked to the S locus and produces two transcripts expressed predominantly in anthers. SPA alleles exhibit a degree of sequence polymorphism comparable to that characteristic of the S locus glycoprotein and S receptor kinase genes which are known to be required for self-incompatibility in the stigma. Thus, SPA fulfills several of the criteria predicted for a gene that encodes the pollen component of self-incompatibility. However, analysis of an S-locus deletion that encompasses this gene rules out a role for this gene as a determinant of specificity in the self-incompatibility response. Springer-Verlag Berlin Heidelberg, 2000 Key words Anther genes nationallicence Brassica nationallicence S-locus deletions nationallicence Self-incompatibility nationallicence Casselman A. L. Department of Plant Biology, Cornell University, Ithaca, NY 14853, USA e-mail: jbn2@cornell.edu Tel.: +1-607-255-8189, Fax: +1-607-255-5407, US aut Nasrallah M. E. Department of Plant Biology, Cornell University, Ithaca, NY 14853, USA e-mail: jbn2@cornell.edu Tel.: +1-607-255-8189, Fax: +1-607-255-5407, US aut Nasrallah J. B. Department of Plant Biology, Cornell University, Ithaca, NY 14853, USA e-mail: jbn2@cornell.edu Tel.: +1-607-255-8189, Fax: +1-607-255-5407, US aut https://doi.org/10.1007/s004970050005 text/html Onlinezugriff via DOI 1 research-article jats NATIONALLICENCE 856 40 https://doi.org/10.1007/s004970050005 text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Casselman A. L. Department of Plant Biology, Cornell University, Ithaca, NY 14853, USA e-mail: jbn2@cornell.edu Tel.: +1-607-255-8189, Fax: +1-607-255-5407, US aut NATIONALLICENCE 700 1- Nasrallah M. E. Department of Plant Biology, Cornell University, Ithaca, NY 14853, USA e-mail: jbn2@cornell.edu Tel.: +1-607-255-8189, Fax: +1-607-255-5407, US aut NATIONALLICENCE 700 1- Nasrallah J. B. Department of Plant Biology, Cornell University, Ithaca, NY 14853, USA e-mail: jbn2@cornell.edu Tel.: +1-607-255-8189, Fax: +1-607-255-5407, US aut Metadata rights reserved Springer special CC-BY-NC licence nationallicence BK010053 XK010053 XK010000 NATIONALLICENCE NATIONALLICENCE NL-springer