caa a22 4500 475817508 CHVBK 20180406123813.0 cr unu---uuuuu 170329e20000101xx s 000 0 eng 10.1007/s004970050008 doi (NATIONALLICENCE)springer-10.1007/s004970050008 A new procedure to asses pollen viability [Elektronische Daten] [T. Rodriguez-Riano, A. Dafni] Abstract : We tested pollen viability of eight species using four vital dyes, a new peroxidase test together with three other established methods (MTT, Baker's and X-Gal), to determine their potential to differentiate fresh pollen from pollen heated for 2 h and 24 h at 80°C (killed pollen) and compared the results with in vitro germination. We found that two of three dyes previously employed to determine viability also stained killed pollen, while the new peroxidase test and MTT did not. We suggest that the latter two are the best methods to test pollen viability, since they do not normally stain either killed or aborted pollen. Springer-Verlag Berlin Heidelberg, 2000 Key words Pollen germination nationallicence MTT nationallicence Peroxidase nationallicence Rodriguez-Riano T. Institute of Evolution, Haifa University, Haifa 31905, Israel e-mail: adafni@research.haifa.ac.il Fax: +972-4-8240312, IL aut Dafni A. Institute of Evolution, Haifa University, Haifa 31905, Israel e-mail: adafni@research.haifa.ac.il Fax: +972-4-8240312, IL aut https://doi.org/10.1007/s004970050008 text/html Onlinezugriff via DOI 1 research-article jats NATIONALLICENCE 856 40 https://doi.org/10.1007/s004970050008 text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Rodriguez-Riano T. Institute of Evolution, Haifa University, Haifa 31905, Israel e-mail: adafni@research.haifa.ac.il Fax: +972-4-8240312, IL aut NATIONALLICENCE 700 1- Dafni A. Institute of Evolution, Haifa University, Haifa 31905, Israel e-mail: adafni@research.haifa.ac.il Fax: +972-4-8240312, IL aut Metadata rights reserved Springer special CC-BY-NC licence nationallicence BK010053 XK010053 XK010000 NATIONALLICENCE NATIONALLICENCE NL-springer