caa a22 4500 475817532 CHVBK 20180406123813.0 cr unu---uuuuu 170329e20000401xx s 000 0 eng 10.1007/s004970050197 doi (NATIONALLICENCE)springer-10.1007/s004970050197 Comparison of transient and stable expression by a pollen-specific promoter: the transformation results do not always agree [Elektronische Daten] [D. A. Hamilton, Y. H. Schwarz, J. Rueda, J. P. Mascarenhas] Abstract : In the analysis of 5' sequence elements necessary for promoter activity in pollen various promoter- reporter gene constructs usually show similar patterns of expression in pollen when assayed by either transient transformations or after stable transformations in transgenic plants. Out of several fragment lengths of the Zm13 pollen-specific promoter linked to GUS, we found that the largest fragment (-1001 to +61) showed approximately eightfold higher expression in pollen of transgenic plants than transient transformations by microprojectile bombardment. No other promoter fragment tested showed this difference. Titration of a limited number of transcription factors by the large number of potential binding sites in this promoter fragment along with the large number of promoter copies introduced during transient transformation are possible explanations for this observation. Springer-Verlag Berlin Heidelberg, 2000 Key words Zm13 pollen-specific promoter nationallicence Transient transformation nationallicence Transgenic plants nationallicence Transcription factor competition nationallicence Hamilton D. A. Department of Biology, Hartwick College, Oneonta, NY 13820, USA, US aut Schwarz Y. H. Department of Biological Sciences and Center for Molecular Genetics, University at Albany, State University of New York, Albany, New York 12222, USA, US aut Rueda J. Departamento de Genética, Facultad de Biología, Universidad Complutense de Madrid, 28040 Madrid, Spain, ES aut Mascarenhas J. P. Department of Biological Sciences and Center for Molecular Genetics, University at Albany, State University of New York, Albany, New York 12222, USA, US aut https://doi.org/10.1007/s004970050197 text/html Onlinezugriff via DOI 1 research-article jats NATIONALLICENCE 856 40 https://doi.org/10.1007/s004970050197 text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Hamilton D. A. Department of Biology, Hartwick College, Oneonta, NY 13820, USA, US aut NATIONALLICENCE 700 1- Schwarz Y. H. Department of Biological Sciences and Center for Molecular Genetics, University at Albany, State University of New York, Albany, New York 12222, USA, US aut NATIONALLICENCE 700 1- Rueda J. Departamento de Genética, Facultad de Biología, Universidad Complutense de Madrid, 28040 Madrid, Spain, ES aut NATIONALLICENCE 700 1- Mascarenhas J. P. Department of Biological Sciences and Center for Molecular Genetics, University at Albany, State University of New York, Albany, New York 12222, USA, US aut Metadata rights reserved Springer special CC-BY-NC licence nationallicence BK010053 XK010053 XK010000 NATIONALLICENCE NATIONALLICENCE NL-springer