caa a22 4500 475817540 CHVBK 20180406123813.0 cr unu---uuuuu 170329e20000401xx s 000 0 eng 10.1007/s004970050198 doi (NATIONALLICENCE)springer-10.1007/s004970050198 Unequal distribution of DNA-containing organelles in generative and sperm cells of Erythrina crista-galli (Fabaceae) [Elektronische Daten] [C. Saito, N. Nagata, A. Sakai, K. Mori, H. Kuroiwa, T. Kuroiwa] Abstract : The generative cell at anthesis in the mature pollen grain of Erythrina crista-galli (Fabaceae) was examined by 4,6-diamidino-2-phenylindole(DAPI)-fluorescence microscopy using the squash method. An unequal, polarized distribution of DNA-containing organelles (plastids and/or mitochondria) within the generative cell was observed in every mature pollen grain examined. Polarization of DNA-containing organelles is obvious when generative cells are freed and assume a spherical shape soon after microspore mitosis, as revealed by fluorescence-microscopic observations of specimens embedded in Technovit 7100 resin and thin-sectioned at different developmental stages. Early establishment of polarized localization of organelles in young generative cells of E. crista-galli and maintenance of this unequal distribution until pollen maturation strongly suggests that the organelles may still be clustered at pollen mitosis. Production of a dimorphic pair of sperm cells, as has been reported in Plumbago zeylanica, was observed in some pollen tubes germinated in vitro. The differentiation of the two sperm cells is discussed in relation to possible preferential double fertilization in angiosperms. Springer-Verlag Berlin Heidelberg, 2000 Key words Erythrina nationallicence Generative cell nationallicence DNA-containing organelle nationallicence Plumbago nationallicence Preferential double fertilization nationallicence Sperm cell nationallicence Saito C. Department of Biological Sciences, Graduate School of Science, University of Tokyo, Hongo 7-3-1, Tokyo, 113-0033, Japan, JP aut Nagata N. Institute of Physical and Chemical Research (RIKEN), 2-1 Hirosawa, Wako, Saitama 351-0198, Japan, JP aut Sakai A. Department of Biological Sciences, Graduate School of Science, University of Tokyo, Hongo 7-3-1, Tokyo, 113-0033, Japan, JP aut Mori K. Department of Biological Sciences, Graduate School of Science, University of Tokyo, Hongo 7-3-1, Tokyo, 113-0033, Japan, JP aut Kuroiwa H. Department of Biological Sciences, Graduate School of Science, University of Tokyo, Hongo 7-3-1, Tokyo, 113-0033, Japan, JP aut Kuroiwa T. Department of Biological Sciences, Graduate School of Science, University of Tokyo, Hongo 7-3-1, Tokyo, 113-0033, Japan, JP aut https://doi.org/10.1007/s004970050198 text/html Onlinezugriff via DOI 1 research-article jats NATIONALLICENCE 856 40 https://doi.org/10.1007/s004970050198 text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Saito C. Department of Biological Sciences, Graduate School of Science, University of Tokyo, Hongo 7-3-1, Tokyo, 113-0033, Japan, JP aut NATIONALLICENCE 700 1- Nagata N. Institute of Physical and Chemical Research (RIKEN), 2-1 Hirosawa, Wako, Saitama 351-0198, Japan, JP aut NATIONALLICENCE 700 1- Sakai A. Department of Biological Sciences, Graduate School of Science, University of Tokyo, Hongo 7-3-1, Tokyo, 113-0033, Japan, JP aut NATIONALLICENCE 700 1- Mori K. Department of Biological Sciences, Graduate School of Science, University of Tokyo, Hongo 7-3-1, Tokyo, 113-0033, Japan, JP aut NATIONALLICENCE 700 1- Kuroiwa H. Department of Biological Sciences, Graduate School of Science, University of Tokyo, Hongo 7-3-1, Tokyo, 113-0033, Japan, JP aut NATIONALLICENCE 700 1- Kuroiwa T. Department of Biological Sciences, Graduate School of Science, University of Tokyo, Hongo 7-3-1, Tokyo, 113-0033, Japan, JP aut Metadata rights reserved Springer special CC-BY-NC licence nationallicence BK010053 XK010053 XK010000 NATIONALLICENCE NATIONALLICENCE NL-springer