caa a22 4500 475817567 CHVBK 20180406123813.0 cr unu---uuuuu 170329e20000401xx s 000 0 eng 10.1007/s004970050195 doi (NATIONALLICENCE)springer-10.1007/s004970050195 The pollen-specific gene Ntp303 encodes a 69-kDa glycoprotein associated with the vegetative membranes and the cell wall [Elektronische Daten] [F. R. A. Wittink, B. Knuiman, J. Derksen, V. Čapková, D. Twell, J. A. M. Schrauwen, George J. Wullems] Abstract : The pollen-specific gene Ntp303 belongs to the class of late pollen specific genes. It is first transcribed directly after pollen mitosis. Biochemical properties, appearance and precise location of the NTP303 protein during pollen development and pollen tube growth were studied by amino-acid micro-sequencing, protein gel blotting and immuno-localization. Antisera were raised against recombinant proteins, encoded by sequences of the pollen-specific Ntp303 gene. The antibodies specifically recognized a 69-kDa glycoprotein. Electron-microscopic immuno-localization of the protein revealed the presence of high concentrations of the NTP303 protein at the vegetative plasma membranes that surround the vegetative cell, the generative cell and the sperm cells of pollen and pollen tubes. The generative plasma membranes of the generative cell and the sperm cells were negative. NTP303 protein was also present in the cell walls and in callose plugs. With this method it was shown that the NTP303 protein was already present in mid-bicellular pollen, after the first, asymmetrical pollen mitosis. Possible functions for the NTP303 protein are discussed in relation to its properties and its association with the vegetative plasma membranes. Springer-Verlag Berlin Heidelberg, 2000 Key words NTP303 glycoprotein nationallicence Pollen-specific Ntp303 gene nationallicence Pollen-tube specific protein nationallicence Plant reproduction nationallicence Biochemical analysis nationallicence Pollen development nationallicence Wittink F. R. A. RijkZwaan, Vrouwgelenweg 90, NL-3341BT Hendrik Ido Ambacht, The Netherlands, NL aut Knuiman B. Department of Experimental Botany, University of Nijmegen, Toernooiveld 1, NL-6525 ED Nijmegen, The Netherlands, e-mail: wullems@sci.kun.nl Tel.: +31-24-3652688, Fax +31-24-2553450, NL aut Derksen J. Department of Experimental Botany, University of Nijmegen, Toernooiveld 1, NL-6525 ED Nijmegen, The Netherlands, e-mail: wullems@sci.kun.nl Tel.: +31-24-3652688, Fax +31-24-2553450, NL aut Čapková V. Institute of Experimental Botany, Academy of Sciences of Czech Republic, Laboratory of Pollen Biology, Rozvojova 135, Prague 6, 16502, Czech Republic, CZ aut Twell D. Department of Biology, University of Leicester, Leicester LE 1 7RH, United Kingdom, GB aut Schrauwen J. A. M. Department of Experimental Botany, University of Nijmegen, Toernooiveld 1, NL-6525 ED Nijmegen, The Netherlands, e-mail: wullems@sci.kun.nl Tel.: +31-24-3652688, Fax +31-24-2553450, NL aut Wullems George J. Department of Experimental Botany, University of Nijmegen, Toernooiveld 1, NL-6525 ED Nijmegen, The Netherlands, e-mail: wullems@sci.kun.nl Tel.: +31-24-3652688, Fax +31-24-2553450, NL aut https://doi.org/10.1007/s004970050195 text/html Onlinezugriff via DOI 1 research-article jats NATIONALLICENCE 856 40 https://doi.org/10.1007/s004970050195 text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Wittink F. R. A. RijkZwaan, Vrouwgelenweg 90, NL-3341BT Hendrik Ido Ambacht, The Netherlands, NL aut NATIONALLICENCE 700 1- Knuiman B. Department of Experimental Botany, University of Nijmegen, Toernooiveld 1, NL-6525 ED Nijmegen, The Netherlands, e-mail: wullems@sci.kun.nl Tel.: +31-24-3652688, Fax +31-24-2553450, NL aut NATIONALLICENCE 700 1- Derksen J. Department of Experimental Botany, University of Nijmegen, Toernooiveld 1, NL-6525 ED Nijmegen, The Netherlands, e-mail: wullems@sci.kun.nl Tel.: +31-24-3652688, Fax +31-24-2553450, NL aut NATIONALLICENCE 700 1- Čapková V. Institute of Experimental Botany, Academy of Sciences of Czech Republic, Laboratory of Pollen Biology, Rozvojova 135, Prague 6, 16502, Czech Republic, CZ aut NATIONALLICENCE 700 1- Twell D. Department of Biology, University of Leicester, Leicester LE 1 7RH, United Kingdom, GB aut NATIONALLICENCE 700 1- Schrauwen J. A. M. Department of Experimental Botany, University of Nijmegen, Toernooiveld 1, NL-6525 ED Nijmegen, The Netherlands, e-mail: wullems@sci.kun.nl Tel.: +31-24-3652688, Fax +31-24-2553450, NL aut NATIONALLICENCE 700 1- Wullems George J. Department of Experimental Botany, University of Nijmegen, Toernooiveld 1, NL-6525 ED Nijmegen, The Netherlands, e-mail: wullems@sci.kun.nl Tel.: +31-24-3652688, Fax +31-24-2553450, NL aut Metadata rights reserved Springer special CC-BY-NC licence nationallicence BK010053 XK010053 XK010000 NATIONALLICENCE NATIONALLICENCE NL-springer