caa a22 4500 475817591 CHVBK 20180406123813.0 cr unu---uuuuu 170329e20000401xx s 000 0 eng 10.1007/s004970050194 doi (NATIONALLICENCE)springer-10.1007/s004970050194 In vitro double fertilization in Nicotiana tabacum (L.): fusion behavior and gamete interaction traced by video-enhanced microscopy [Elektronische Daten] [M. -X. Sun, A. Moscatelli, H. -Y. Yang, M. Cresti] Abstract : In vitro double fertilization in tobacco was carried out with attention to fusion behavior and gamete interaction. Structural and cytological events indicating possible reaction to the fusion of sperm-egg and especially sperm-central cell were recorded by video-enhanced microscopy. Generative cells were fused with the egg cell or central cell as a control system to better understand gamete interaction. As early as adherence of the male cell, the female cell showed response by means of cytoplasm strand formation. After gamete fusion, cytoplasm activation in the egg cell was observed as long distance movement of organelles. In fertilized central cells, however, fusion did not result in notable cytological change within 30 min. Male nuclear movement recorded in the female cell illustrated two different patterns of movement which showed similarity to organelle movement. The dynamics of male and female nuclear fusion after in vitro fertilization was also recorded in the central cell. It revealed that the fusion process requires only a few seconds and is similar to that of gamete fusion in vitro. This may offer a new clue for understanding how female and male nuclei attract, adhere and finally fuse each other. Springer-Verlag Berlin Heidelberg, 2000 Key words Nicotiana tabacum nationallicence In vitro fertilization nationallicence Gamete interaction nationallicence Cytology nationallicence Sun M. -X Research Center for Developmental Biology, Wuhan University, Wuhan, China, CN aut Moscatelli A. Institute of Botany, Messina University, Salita Sperone 31, 98165 Messina, Italy, IT aut Yang H. -Y Research Center for Developmental Biology, Wuhan University, Wuhan, China, CN aut Cresti M. Department of Environmental Science, Siena University, 53100 Siena, Italy, e-mail: cresti@unisi.it Fax: +39-577-232860, Tel.: +39-577-232854, IT aut https://doi.org/10.1007/s004970050194 text/html Onlinezugriff via DOI 1 research-article jats NATIONALLICENCE 856 40 https://doi.org/10.1007/s004970050194 text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Sun M. -X Research Center for Developmental Biology, Wuhan University, Wuhan, China, CN aut NATIONALLICENCE 700 1- Moscatelli A. Institute of Botany, Messina University, Salita Sperone 31, 98165 Messina, Italy, IT aut NATIONALLICENCE 700 1- Yang H. -Y Research Center for Developmental Biology, Wuhan University, Wuhan, China, CN aut NATIONALLICENCE 700 1- Cresti M. Department of Environmental Science, Siena University, 53100 Siena, Italy, e-mail: cresti@unisi.it Fax: +39-577-232860, Tel.: +39-577-232854, IT aut Metadata rights reserved Springer special CC-BY-NC licence nationallicence BK010053 XK010053 XK010000 NATIONALLICENCE NATIONALLICENCE NL-springer