caa a22 4500 475817613 CHVBK 20180406123813.0 cr unu---uuuuu 170329e20000701xx s 000 0 eng 10.1007/s004970000036 doi (NATIONALLICENCE)springer-10.1007/s004970000036 Characterization and function of wall-bound exo-β-glucanases of Lilium longiflorum pollen tubes [Elektronische Daten] [Toshihisa Kotake, Y.-Q. Li, Mai Takahashi, N. Sakurai] Abstract : Two exo-β-glucanases (LP-ExoI, 83 kDa and LP-ExoII, 71 kDa) were extracted and partially purified from the cell wall of Lilium longiflorum pollen tubes. Both LP-ExoI and LP-ExoII hydrolyzed laminarin (1,3-β-glucan). These enzymes also exhibited some activity toward 1,3:1,4-β-glucans of Hordeum vulgare and Cetraria islandica and the 1,6-β-glucan of Umbilicaria papullosa. The pH for optimum activity for both exo-β-glucanases was 5.5. Methylation analysis of the reaction products revealed that purified LP-ExoI decreased both 1,3- and 1,4-glucosyl linkages in hemicellulosic polysaccharides isolated from the cell wall of lily pollen tubes. D-gluconolactone and nojirimycin, inhibitors of glucosidase, inhibited activities of both exo-β-glucanases, as well as growth of the lily pollen tubes. These results disclosed that the wall-bound exo-β-glucanases play an important role in the regulation of lily pollen tube growth. Springer-Verlag Berlin Heidelberg, 2000 Key words 1 nationallicence 3-β-glucan/1 nationallicence 4-β-glucan nationallicence Cell Wall nationallicence Exo-β-glucanase nationallicence Lily (Lilium longiflorum) nationallicence Pollen tube growth nationallicence Kotake Toshihisa Department of Environmental Studies, Faculty of Integrated Arts and Sciences, Hiroshima University, Higashi-Hiroshima 739, Japan e-mail: nsakura@hiroshima-u.ac.jp; Tel. +81-824-24-6515, Fax +81-824-24-0758, JP aut Li Y.-Q Department of Biological Sciences and Biotechnology, Tsinghua University, Beijing, China, CN aut Takahashi Mai aut Sakurai N. Department of Environmental Studies, Faculty of Integrated Arts and Sciences, Hiroshima University, Higashi-Hiroshima 739, Japan e-mail: nsakura@hiroshima-u.ac.jp; Tel. +81-824-24-6515, Fax +81-824-24-0758, JP aut https://doi.org/10.1007/s004970000036 text/html Onlinezugriff via DOI 1 research-article jats NATIONALLICENCE 856 40 https://doi.org/10.1007/s004970000036 text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Kotake Toshihisa Department of Environmental Studies, Faculty of Integrated Arts and Sciences, Hiroshima University, Higashi-Hiroshima 739, Japan e-mail: nsakura@hiroshima-u.ac.jp; Tel. +81-824-24-6515, Fax +81-824-24-0758, JP aut NATIONALLICENCE 700 1- Li Y.-Q Department of Biological Sciences and Biotechnology, Tsinghua University, Beijing, China, CN aut NATIONALLICENCE 700 1- Takahashi Mai aut NATIONALLICENCE 700 1- Sakurai N. Department of Environmental Studies, Faculty of Integrated Arts and Sciences, Hiroshima University, Higashi-Hiroshima 739, Japan e-mail: nsakura@hiroshima-u.ac.jp; Tel. +81-824-24-6515, Fax +81-824-24-0758, JP aut Metadata rights reserved Springer special CC-BY-NC licence nationallicence BK010053 XK010053 XK010000 NATIONALLICENCE NATIONALLICENCE NL-springer