caa a22 4500 475817621 CHVBK 20180406123813.0 cr unu---uuuuu 170329e20000701xx s 000 0 eng 10.1007/PL00009838 doi (NATIONALLICENCE)springer-10.1007/PL00009838 The MADS box gene ZmMADS 2 is specifically expressed in maize pollen and during maize pollen tube growth [Elektronische Daten] [Sigrid Heuer, Horst Lörz, T. Dresselhaus] Abstract : We screened a cDNA library of mature maize pollen for clones encoding transcription factors. Two novel cDNAs (ZmMADS1 and ZmMADS2) encoding MADS box proteins were isolated. To our knowledge, ZmMADS1 and ZmMADS2 represent the first transcription factors isolated from pollen of a monocot plant species. ZmMADS1 can be classified as a member of the TM3 subfamily of MADS box proteins whereas ZmMADS2, which is described here in more detail, belongs to the AGL17 subfamily. Temporal and spatial expression analyses showed that ZmMADS2 expression is restricted to pollen and roots. During pollen development ZmMADS2 is detectable at all stages of pollen development analyzed by RT-PCR but is most abundant in mature pollen after dehiscence. The establishment of a whole-mount RNA in situ hybridization protocol with in-vitro germinated pollen enabled the localization of ZmMADS2 transcripts in pollen tubes. ZmMADS2 transcripts are translocated into pollen tubes immediately upon germination and display a gradient with increasing concentration towards the tube tip. The vegetative nucleus and the sperm cells, putative targets of ZmMADS2, enter the pollen tube about 2 h later. Springer-Verlag Berlin Heidelberg, 2000 Key words MADS box nationallicence Maize pollen nationallicence Pollen tube nationallicence Gene regulation nationallicence Male germ unit nationallicence Heuer Sigrid Applied Plant Molecular Biology II; University of Hamburg, Ohnhorststrasse 18, 22609 Hamburg, Germany e-mail: dresselh@botanik.uni-hamburg.de; Tel.: +49-40-42816-360/416, Fax: +49-40-42816-229, DE aut Lörz Horst Applied Plant Molecular Biology II; University of Hamburg, Ohnhorststrasse 18, 22609 Hamburg, Germany e-mail: dresselh@botanik.uni-hamburg.de; Tel.: +49-40-42816-360/416, Fax: +49-40-42816-229, DE aut Dresselhaus T. Applied Plant Molecular Biology II; University of Hamburg, Ohnhorststrasse 18, 22609 Hamburg, Germany e-mail: dresselh@botanik.uni-hamburg.de; Tel.: +49-40-42816-360/416, Fax: +49-40-42816-229, DE aut https://doi.org/10.1007/PL00009838 text/html Onlinezugriff via DOI 1 research-article jats NATIONALLICENCE 856 40 https://doi.org/10.1007/PL00009838 text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Heuer Sigrid Applied Plant Molecular Biology II; University of Hamburg, Ohnhorststrasse 18, 22609 Hamburg, Germany e-mail: dresselh@botanik.uni-hamburg.de; Tel.: +49-40-42816-360/416, Fax: +49-40-42816-229, DE aut NATIONALLICENCE 700 1- Lörz Horst Applied Plant Molecular Biology II; University of Hamburg, Ohnhorststrasse 18, 22609 Hamburg, Germany e-mail: dresselh@botanik.uni-hamburg.de; Tel.: +49-40-42816-360/416, Fax: +49-40-42816-229, DE aut NATIONALLICENCE 700 1- Dresselhaus T. Applied Plant Molecular Biology II; University of Hamburg, Ohnhorststrasse 18, 22609 Hamburg, Germany e-mail: dresselh@botanik.uni-hamburg.de; Tel.: +49-40-42816-360/416, Fax: +49-40-42816-229, DE aut Metadata rights reserved Springer special CC-BY-NC licence nationallicence BK010053 XK010053 XK010000 NATIONALLICENCE NATIONALLICENCE NL-springer