caa a22 4500 47581763X CHVBK 20180406123813.0 cr unu---uuuuu 170329e20000701xx s 000 0 eng 10.1007/PL00009837 doi (NATIONALLICENCE)springer-10.1007/PL00009837 Calcium changes in ovules and embryo sacs of Plumbago zeylanica L [Elektronische Daten] [H. Q. Tian, H. Zhu, S. D. Russell] Abstract : Calcium was localized in ovules of Plumbago zeylanica from 1 day before anthesis to 3 days after anthesis using potassium antimonate and transmission electron microscopy in pollinated and emasculated flowers. At 1 day before anthesis, embryo sacs (containing an egg cell, a central cell and zero to three accessory cells) appear mature and contain abundant calcium precipitates (ppts), in contrast to nucellar cells. At anthesis, the vacuoles of nucellar cells have enlarged, and micropylar cells, in particular, are heavily labeled with calcium ppts. As pollen tubes elongate through ovular tissues, ppts diminish in ovular cells and become concentrated in the pollen tube cell wall. After fertilization, the calcium ppts sharply diminish in fertilized ovules; in unfertilized ovules, calcium ppts remain abundant up to 3 days after anthesis (when unfertilized ovules are shed). The distribution of calcium in the ovule changes in apparent response to fertilization, suggesting that calcium content may be related to the attraction and receipt of the pollen tube. In contrast with conventionally-organized embryo sacs with synergids, Plumbago accumulates calcium in the egg cell. Springer-Verlag Berlin Heidelberg, 2000 Key words Antimonate localization nationallicence Calcium nationallicence Embryo sac nationallicence Ovule nationallicence Plumbago zeylanica nationallicence Tian H. Q. Department of Botany and Microbiology, University of Oklahoma, Norman, OK 73019-0245, USA e-mail: srussell@ou.edu Tel.: +1-405-325-6234, Fax: +1-405-325-7619, US aut Zhu H. Department of Botany and Microbiology, University of Oklahoma, Norman, OK 73019-0245, USA e-mail: srussell@ou.edu Tel.: +1-405-325-6234, Fax: +1-405-325-7619, US aut Russell S. D. Department of Botany and Microbiology, University of Oklahoma, Norman, OK 73019-0245, USA e-mail: srussell@ou.edu Tel.: +1-405-325-6234, Fax: +1-405-325-7619, US aut https://doi.org/10.1007/PL00009837 text/html Onlinezugriff via DOI 1 research-article jats NATIONALLICENCE 856 40 https://doi.org/10.1007/PL00009837 text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Tian H. Q. Department of Botany and Microbiology, University of Oklahoma, Norman, OK 73019-0245, USA e-mail: srussell@ou.edu Tel.: +1-405-325-6234, Fax: +1-405-325-7619, US aut NATIONALLICENCE 700 1- Zhu H. Department of Botany and Microbiology, University of Oklahoma, Norman, OK 73019-0245, USA e-mail: srussell@ou.edu Tel.: +1-405-325-6234, Fax: +1-405-325-7619, US aut NATIONALLICENCE 700 1- Russell S. D. Department of Botany and Microbiology, University of Oklahoma, Norman, OK 73019-0245, USA e-mail: srussell@ou.edu Tel.: +1-405-325-6234, Fax: +1-405-325-7619, US aut Metadata rights reserved Springer special CC-BY-NC licence nationallicence BK010053 XK010053 XK010000 NATIONALLICENCE NATIONALLICENCE NL-springer