caa a22 4500 475817648 CHVBK 20180406123813.0 cr unu---uuuuu 170329e20000701xx s 000 0 eng 10.1007/PL00009841 doi (NATIONALLICENCE)springer-10.1007/PL00009841 Nuclear pore dynamics during pollen development and androgenesis in Brassica napus [Elektronische Daten] [K. R. Straatman, J. Nijsse, H. Kieft, A. C. van Aelst, J. H. N. Schel] Abstract : Changes in nuclear pore complex (NPC) densities, NPCs/nucleus and NPCs/µm3, are described using freeze-fractured Brassica napus microspores and pollen in vivo and in vitro. Early stages of microspore- and pollen-derived embryogenic cells were also analysed. The results of in vivo and in vitro pollen development indicate an increase in activity of the vegetative nucleus during maturation of the pollen. At the onset of microspore and pollen culture, NPC density decreased from 15 NPCs/µm2 at the stage of isolation to 9 NPCs/µm2, under both embryogenic and non-embryogenic conditions. This implies that the drop in NPC density might be a result of culturing the microspores and pollen rather than an indication for microspore and pollen embryogenesis in Brassica napus. However, after 1 day in culture under embryogenic conditions, the NPC density increased again and stabilised around 13 NPCs/µm2, whereas under non-embryogenic conditions the NPC density remained about 9 NPCs/µm2. This low density of 9 NPCs/µm2 was also found in the nuclei of sperm cells, in contrast to the 19 NPCs/µm2 found in the vegetative nucleus. It means that, although both the vegetative and sperm nuclei are believed to be metabolically rather inactive in mature pollen, the NPC density of vegetative nucleus is twice as high as the NPC density of the sperm nuclei. In a few cases, embryos formed suspensor-like structures with a NPC density of 9 NPCs/µm2, indicating a lower nucleocytoplasmic exchange of the nuclei of the suspensor cells than with the nuclei in the embryo proper. In addition, observations on NPCs and other organelles, obtained by high resolution cryo-scanning microscopy, are presented. Springer-Verlag Berlin Heidelberg, 2000 Key words Brassica napus nationallicence Androgenesis nationallicence Freeze fracturing nationallicence Nuclear pore complex nationallicence Pollen development nationallicence Straatman K. R. Laboratory of Experimental Plant Morphology and Cell Biology, Wageningen University, Arboretumlaan 4, 6703 BD, Wageningen, The Netherlands e-mail: jan.schel@algem.pcm.wau.nl Tel.: +31-317-483848, Fax: +31-317-485005, NL aut Nijsse J. Laboratory of Experimental Plant Morphology and Cell Biology, Wageningen University, Arboretumlaan 4, 6703 BD, Wageningen, The Netherlands e-mail: jan.schel@algem.pcm.wau.nl Tel.: +31-317-483848, Fax: +31-317-485005, NL aut Kieft H. Laboratory of Experimental Plant Morphology and Cell Biology, Wageningen University, Arboretumlaan 4, 6703 BD, Wageningen, The Netherlands e-mail: jan.schel@algem.pcm.wau.nl Tel.: +31-317-483848, Fax: +31-317-485005, NL aut van Aelst A. C. Laboratory of Experimental Plant Morphology and Cell Biology, Wageningen University, Arboretumlaan 4, 6703 BD, Wageningen, The Netherlands e-mail: jan.schel@algem.pcm.wau.nl Tel.: +31-317-483848, Fax: +31-317-485005, NL aut Schel J. H. N. Laboratory of Experimental Plant Morphology and Cell Biology, Wageningen University, Arboretumlaan 4, 6703 BD, Wageningen, The Netherlands e-mail: jan.schel@algem.pcm.wau.nl Tel.: +31-317-483848, Fax: +31-317-485005, NL aut https://doi.org/10.1007/PL00009841 text/html Onlinezugriff via DOI 1 research-article jats NATIONALLICENCE 856 40 https://doi.org/10.1007/PL00009841 text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Straatman K. R. Laboratory of Experimental Plant Morphology and Cell Biology, Wageningen University, Arboretumlaan 4, 6703 BD, Wageningen, The Netherlands e-mail: jan.schel@algem.pcm.wau.nl Tel.: +31-317-483848, Fax: +31-317-485005, NL aut NATIONALLICENCE 700 1- Nijsse J. Laboratory of Experimental Plant Morphology and Cell Biology, Wageningen University, Arboretumlaan 4, 6703 BD, Wageningen, The Netherlands e-mail: jan.schel@algem.pcm.wau.nl Tel.: +31-317-483848, Fax: +31-317-485005, NL aut NATIONALLICENCE 700 1- Kieft H. Laboratory of Experimental Plant Morphology and Cell Biology, Wageningen University, Arboretumlaan 4, 6703 BD, Wageningen, The Netherlands e-mail: jan.schel@algem.pcm.wau.nl Tel.: +31-317-483848, Fax: +31-317-485005, NL aut NATIONALLICENCE 700 1- van Aelst A. C. Laboratory of Experimental Plant Morphology and Cell Biology, Wageningen University, Arboretumlaan 4, 6703 BD, Wageningen, The Netherlands e-mail: jan.schel@algem.pcm.wau.nl Tel.: +31-317-483848, Fax: +31-317-485005, NL aut NATIONALLICENCE 700 1- Schel J. H. N. Laboratory of Experimental Plant Morphology and Cell Biology, Wageningen University, Arboretumlaan 4, 6703 BD, Wageningen, The Netherlands e-mail: jan.schel@algem.pcm.wau.nl Tel.: +31-317-483848, Fax: +31-317-485005, NL aut Metadata rights reserved Springer special CC-BY-NC licence nationallicence BK010053 XK010053 XK010000 NATIONALLICENCE NATIONALLICENCE NL-springer