caa a22 4500 475817664 CHVBK 20180406123813.0 cr unu---uuuuu 170329e20000701xx s 000 0 eng 10.1007/PL00009839 doi (NATIONALLICENCE)springer-10.1007/PL00009839 A Brassica napus skp1 -like gene promoter drives GUS expression in Arabidopsis thaliana male and female gametophytes [Elektronische Daten] [J. Drouaud, Katia Marrocco, Céline Ridel, Georges Pelletier, P. Guerche] Abstract : We isolated a gene, BnSKP1γ1, expressed in rapeseed (Brassica napus) microspores, which encodes a protein closely related to the Saccharomyces cerevisiae Skp1p protein previously shown to play a role in cell cycle regulation. Twelve SKP1-related genes have already been identified in the Arabidopsis thaliana genome. Using a PCR-based strategy, we isolated three other genes. To date, most data available concerning the function of the SKP1-related genes in plants are indirect. Studies on transgenic A. thaliana plants showthat a 1100-bp BnSKP1γ1 promoter fragment can direct GUS expression in female gametophytes soon after the first haploid mitosis and in male gametophytes from the tetrade stage. No GUS expression can be detected in sporophytic tissues. RT-PCR experiments suggest that this gene is expressed in a similar way in rapeseed. This is the first reported case of a gene exhibiting such an expression pattern in angiosperms. Springer-Verlag Berlin Heidelberg, 2000 Key words Gene-specific expression nationallicence skp1-like gene nationallicence GUS staining nationallicence Gametophytic expression nationallicence Brassica napus nationallicence Arabidopsis thaliana nationallicence Drouaud J. Station de Génétique et d"Amélioration des Plantes, Institut National de la Recherche Agronomique, Route de Saint Cyr, 78026 Versailles cedex, France e-mail: guerche@versailles.inra.fr Tel.: +33-1-30 83 30 73, Fax: +33-1-30 83 33 19, FR aut Marrocco Katia Station de Génétique et d"Amélioration des Plantes, Institut National de la Recherche Agronomique, Route de Saint Cyr, 78026 Versailles cedex, France e-mail: guerche@versailles.inra.fr Tel.: +33-1-30 83 30 73, Fax: +33-1-30 83 33 19, FR aut Ridel Céline Station de Génétique et d"Amélioration des Plantes, Institut National de la Recherche Agronomique, Route de Saint Cyr, 78026 Versailles cedex, France e-mail: guerche@versailles.inra.fr Tel.: +33-1-30 83 30 73, Fax: +33-1-30 83 33 19, FR aut Pelletier Georges Station de Génétique et d"Amélioration des Plantes, Institut National de la Recherche Agronomique, Route de Saint Cyr, 78026 Versailles cedex, France e-mail: guerche@versailles.inra.fr Tel.: +33-1-30 83 30 73, Fax: +33-1-30 83 33 19, FR aut Guerche P. Station de Génétique et d"Amélioration des Plantes, Institut National de la Recherche Agronomique, Route de Saint Cyr, 78026 Versailles cedex, France e-mail: guerche@versailles.inra.fr Tel.: +33-1-30 83 30 73, Fax: +33-1-30 83 33 19, FR aut https://doi.org/10.1007/PL00009839 text/html Onlinezugriff via DOI 1 research-article jats NATIONALLICENCE 856 40 https://doi.org/10.1007/PL00009839 text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Drouaud J. Station de Génétique et d"Amélioration des Plantes, Institut National de la Recherche Agronomique, Route de Saint Cyr, 78026 Versailles cedex, France e-mail: guerche@versailles.inra.fr Tel.: +33-1-30 83 30 73, Fax: +33-1-30 83 33 19, FR aut NATIONALLICENCE 700 1- Marrocco Katia Station de Génétique et d"Amélioration des Plantes, Institut National de la Recherche Agronomique, Route de Saint Cyr, 78026 Versailles cedex, France e-mail: guerche@versailles.inra.fr Tel.: +33-1-30 83 30 73, Fax: +33-1-30 83 33 19, FR aut NATIONALLICENCE 700 1- Ridel Céline Station de Génétique et d"Amélioration des Plantes, Institut National de la Recherche Agronomique, Route de Saint Cyr, 78026 Versailles cedex, France e-mail: guerche@versailles.inra.fr Tel.: +33-1-30 83 30 73, Fax: +33-1-30 83 33 19, FR aut NATIONALLICENCE 700 1- Pelletier Georges Station de Génétique et d"Amélioration des Plantes, Institut National de la Recherche Agronomique, Route de Saint Cyr, 78026 Versailles cedex, France e-mail: guerche@versailles.inra.fr Tel.: +33-1-30 83 30 73, Fax: +33-1-30 83 33 19, FR aut NATIONALLICENCE 700 1- Guerche P. Station de Génétique et d"Amélioration des Plantes, Institut National de la Recherche Agronomique, Route de Saint Cyr, 78026 Versailles cedex, France e-mail: guerche@versailles.inra.fr Tel.: +33-1-30 83 30 73, Fax: +33-1-30 83 33 19, FR aut Metadata rights reserved Springer special CC-BY-NC licence nationallicence BK010053 XK010053 XK010000 NATIONALLICENCE NATIONALLICENCE NL-springer