caa a22 4500 475817699 CHVBK 20180406123813.0 cr unu---uuuuu 170329e20001101xx s 000 0 eng 10.1007/s004970000047 doi (NATIONALLICENCE)springer-10.1007/s004970000047 The translationally repressed pollen-specific ntp303 mRNA is stored in non-polysomal mRNPs during pollen maturation [Elektronische Daten] [D. Honys, Jonathan P. Combe, David Twell, Vera Capková] Abstract : A tobacco pollen tube glycoprotein, p69 is encoded by the pollen-specific gene ntp303 that is transcribed during pollen development and pollen tube growth, but it is abundantly translated only after pollen germination. To investigate the translational repression of ntp303 mRNA during pollen development the compartmentation of ntp303 mRNA was examined and compared against another transcript (ntp52), which is efficiently translated during pollen maturation. Three subcellular fractions were isolated: a post-polysomal fraction enriched with messenger ribonucleoprotein particles, a polysomal fraction and a novel fraction of EDTA/ puromycin-resistant particles co-sedimentating with polysomes (EPP). At all developmental stages studied, ntp303 mRNA was found to be present in all fractions. Surprisingly, most of the translationally inactive ntp303 mRNA was localised in the polysomal fraction and EPPs, whereas ntp52 mRNA was distributed between the post-polysomal fraction and polysomes but was virtually undetectable in EPPs. This differential mRNA distribution pattern may help to explain the developmentally regulated translational repression of the ntp303 gene during pollen maturation, highlighting a potential role of EPPs. A model of how this differential mRNA compartmentation pattern regulates ntp303 mRNA translation is proposed. Springer-Verlag Berlin Heidelberg, 2000 Keywords Pollen development nationallicence Pollen-specific ntp303 gene nationallicence Stored mRNA nationallicence Subcellular distribution nationallicence Translational regulation nationallicence Translational repression nationallicence Honys D. Institute of Experimental Botany, Academy of Sciences of the Czech Republic, Laboratory of Pollen Biology, Rozvojová 135, 165 02, Praha 6, Czech Republic e-mail: honys@ueb.cas.cz Fax: +420-2-20390461, Tel.: +420-2-20390450 aut Combe Jonathan P. University of Leicester, Department of Biology, University Road, Leicester LE1 7RH, UK, GB aut Twell David University of Leicester, Department of Biology, University Road, Leicester LE1 7RH, UK, GB aut Capková Vera Institute of Experimental Botany, Academy of Sciences of the Czech Republic, Laboratory of Pollen Biology, Rozvojová 135, 165 02, Praha 6, Czech Republic e-mail: honys@ueb.cas.cz Fax: +420-2-20390461, Tel.: +420-2-20390450 aut https://doi.org/10.1007/s004970000047 text/html Onlinezugriff via DOI 1 research-article jats NATIONALLICENCE 856 40 https://doi.org/10.1007/s004970000047 text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Honys D. Institute of Experimental Botany, Academy of Sciences of the Czech Republic, Laboratory of Pollen Biology, Rozvojová 135, 165 02, Praha 6, Czech Republic e-mail: honys@ueb.cas.cz Fax: +420-2-20390461, Tel.: +420-2-20390450 aut NATIONALLICENCE 700 1- Combe Jonathan P. University of Leicester, Department of Biology, University Road, Leicester LE1 7RH, UK, GB aut NATIONALLICENCE 700 1- Twell David University of Leicester, Department of Biology, University Road, Leicester LE1 7RH, UK, GB aut NATIONALLICENCE 700 1- Capková Vera Institute of Experimental Botany, Academy of Sciences of the Czech Republic, Laboratory of Pollen Biology, Rozvojová 135, 165 02, Praha 6, Czech Republic e-mail: honys@ueb.cas.cz Fax: +420-2-20390461, Tel.: +420-2-20390450 aut Metadata rights reserved Springer special CC-BY-NC licence nationallicence BK010053 XK010053 XK010000 NATIONALLICENCE NATIONALLICENCE NL-springer