caa a22 4500 475817702 CHVBK 20180406123813.0 cr unu---uuuuu 170329e20001101xx s 000 0 eng 10.1007/s004970000054 doi (NATIONALLICENCE)springer-10.1007/s004970000054 Molecular cloning and characterization of two tobacco MADS-box genes [Elektronische Daten] [Yan H. Wu, Q. Li, Jin-Song Zhang, Zhifu Zheng, S. Xue, Y. Li] Abstract : We isolated and characterized two MADS-box genes, TOBM1 and TOBM2, from Nicotiana tabacum. The complete cDNA sequences of TOBM1 and TOBM2 encode a 245 and a 242 amino acid protein, respectively. Amino acid sequence alignment and phylogenetic analyses have shown that TOBM1 and TOBM2 are highly related to the MADS-box-containing PFG, SQUA, and AP1 genes. However, expression patterns of TOBM1 and TOBM2 are significantly different from those of previously published MADS-box genes. TOBM1 and TOBM2 are expressed in the inflorescence meristem and all four floral organs. Within the stamen and carpel, TOBM1 and TOBM2 transcripts are present in immature pollen, ovary wall and ovule. TOBM1 is also active in stem tissues. Springer-Verlag Berlin Heidelberg, 2000 Keywords MADS-box genes nationallicence Flower development nationallicence Expression pattern nationallicence Tobacco nationallicence Wu Yan H. Department of Plant Science, University of Connecticut, Storrs CT 06269, USA e-mail: yi.li@uconn.edu Tel.: +1-860-486-6780, Fax: +1-860-486-6777, US aut Li Q. Department of Plant Science, University of Connecticut, Storrs CT 06269, USA e-mail: yi.li@uconn.edu Tel.: +1-860-486-6780, Fax: +1-860-486-6777, US aut Zhang Jin-Song Department of Plant Science, University of Connecticut, Storrs CT 06269, USA e-mail: yi.li@uconn.edu Tel.: +1-860-486-6780, Fax: +1-860-486-6777, US aut Zheng Zhifu Department of Plant Science, University of Connecticut, Storrs CT 06269, USA e-mail: yi.li@uconn.edu Tel.: +1-860-486-6780, Fax: +1-860-486-6777, US aut Xue S. Department of Plant Science, University of Connecticut, Storrs CT 06269, USA e-mail: yi.li@uconn.edu Tel.: +1-860-486-6780, Fax: +1-860-486-6777, US aut Li Y. Department of Plant Science, University of Connecticut, Storrs CT 06269, USA e-mail: yi.li@uconn.edu Tel.: +1-860-486-6780, Fax: +1-860-486-6777, US aut https://doi.org/10.1007/s004970000054 text/html Onlinezugriff via DOI 1 research-article jats NATIONALLICENCE 856 40 https://doi.org/10.1007/s004970000054 text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Wu Yan H. Department of Plant Science, University of Connecticut, Storrs CT 06269, USA e-mail: yi.li@uconn.edu Tel.: +1-860-486-6780, Fax: +1-860-486-6777, US aut NATIONALLICENCE 700 1- Li Q. Department of Plant Science, University of Connecticut, Storrs CT 06269, USA e-mail: yi.li@uconn.edu Tel.: +1-860-486-6780, Fax: +1-860-486-6777, US aut NATIONALLICENCE 700 1- Zhang Jin-Song Department of Plant Science, University of Connecticut, Storrs CT 06269, USA e-mail: yi.li@uconn.edu Tel.: +1-860-486-6780, Fax: +1-860-486-6777, US aut NATIONALLICENCE 700 1- Zheng Zhifu Department of Plant Science, University of Connecticut, Storrs CT 06269, USA e-mail: yi.li@uconn.edu Tel.: +1-860-486-6780, Fax: +1-860-486-6777, US aut NATIONALLICENCE 700 1- Xue S. Department of Plant Science, University of Connecticut, Storrs CT 06269, USA e-mail: yi.li@uconn.edu Tel.: +1-860-486-6780, Fax: +1-860-486-6777, US aut NATIONALLICENCE 700 1- Li Y. Department of Plant Science, University of Connecticut, Storrs CT 06269, USA e-mail: yi.li@uconn.edu Tel.: +1-860-486-6780, Fax: +1-860-486-6777, US aut Metadata rights reserved Springer special CC-BY-NC licence nationallicence BK010053 XK010053 XK010000 NATIONALLICENCE NATIONALLICENCE NL-springer