caa a22 4500 475817710 CHVBK 20180406123813.0 cr unu---uuuuu 170329e20001101xx s 000 0 eng 10.1007/s004970000048 doi (NATIONALLICENCE)springer-10.1007/s004970000048 A self-compatible mutant S allele conferring a dominant negative effect on the functional S allele in Ipomoea trifida [Elektronische Daten] [K. Kakeda, H. Tsukada, Y. Kowyama] Abstract : A spontaneously occurring self-compatible mutant has been identified in Ipomoea trifida, a species possessing sporophytic self-incompatibility controlled by a single multiallelic S locus. Analysis of the segregation of compatibility/incompatibility phenotypes in selfed and crossed progenies of the self-compatible mutant plant indicated that the self-compatibility trait was caused by a mutation at the S locus; the mutated S allele was therefore designated Sc. RFLP analysis of progeny plants segregating for the Sc allele using the SSP gene (a gene linked closely to the S locus of I. trifida) as a probe confirmed that the mutation was present at the S locus. Self-incompatibility responses were examined in F1 progenies obtained from crosses between the self-compatible mutant and self-incompatible plants homozygous for one of three S alleles, S 1 , S 3 and S 22 , where the dominance relationship is S 22 >S 1 >S 3 . All F1 progeny plants from crosses with S 22 and S 1 homozygotes were self-incompatible and exhibited the respective phenotypes of each self-incompatible parent (either S 22 or S 1 ) in both stigma and pollen. However, of the F1 progeny plants from the cross with the S 3 homozygote, those carrying the genotype ScS 3 were all self-compatible and cross-compatible as both female and male parents with the S 3 homozygote. These results indicate that the dominance relationship between the four S alleles is: S 22 >S 1 >Sc>S 3 and so reveal the unexpected finding that the mutated Sc allele is dominant over a functional S 3 allele. A possible explanation for this observation is that the gene product encoded by the Sc allele confers a dominant negative effect on the S 3 gene product. Springer-Verlag Berlin Heidelberg, 2000 Keywords Sporophytic self-incompatibility nationallicence Self-compatible mutant nationallicence S alleles nationallicence Dominance relationships nationallicence Ipomoea trifida nationallicence Kakeda K. Faculty of Bioresources, Mie University, 1515 Kamihama, Tsu 514-8507, Japan e-mail: kakeda@bio.mie-u.ac.jp Fax: +81-59-231 9637, JP aut Tsukada H. Faculty of Bioresources, Mie University, 1515 Kamihama, Tsu 514-8507, Japan e-mail: kakeda@bio.mie-u.ac.jp Fax: +81-59-231 9637, JP aut Kowyama Y. Faculty of Bioresources, Mie University, 1515 Kamihama, Tsu 514-8507, Japan e-mail: kakeda@bio.mie-u.ac.jp Fax: +81-59-231 9637, JP aut https://doi.org/10.1007/s004970000048 text/html Onlinezugriff via DOI 1 research-article jats NATIONALLICENCE 856 40 https://doi.org/10.1007/s004970000048 text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Kakeda K. Faculty of Bioresources, Mie University, 1515 Kamihama, Tsu 514-8507, Japan e-mail: kakeda@bio.mie-u.ac.jp Fax: +81-59-231 9637, JP aut NATIONALLICENCE 700 1- Tsukada H. Faculty of Bioresources, Mie University, 1515 Kamihama, Tsu 514-8507, Japan e-mail: kakeda@bio.mie-u.ac.jp Fax: +81-59-231 9637, JP aut NATIONALLICENCE 700 1- Kowyama Y. Faculty of Bioresources, Mie University, 1515 Kamihama, Tsu 514-8507, Japan e-mail: kakeda@bio.mie-u.ac.jp Fax: +81-59-231 9637, JP aut Metadata rights reserved Springer special CC-BY-NC licence nationallicence BK010053 XK010053 XK010000 NATIONALLICENCE NATIONALLICENCE NL-springer