caa a22 4500 475817729 CHVBK 20180406123813.0 cr unu---uuuuu 170329e20001101xx s 000 0 eng 10.1007/s004970000053 doi (NATIONALLICENCE)springer-10.1007/s004970000053 A novel Brassica napus L. pollen-specific gene belongs to a nucleic-acid-binding protein family [Elektronische Daten] [Petr Sm´ykal, Iveta Janotová, P. Pechan] Abstract : A differential display PCR approach was used to search for up and down regulated genes expressed during pollen development of Brassica napus. One of a number of isolated DNA fragments was shown to be pollen specific. Screening of a pollen cDNA library yielded the full length cDNA Bn 1 clone. The corresponding transcript was first detected around first pollen mitosis and increased in abundance until pollen maturity. The Bn1 RBP clone is 1743 bp long and codes for a polypeptide of 367 amino acids. The Bn1 RBP predicted protein has an estimated molecular weight of 40 kDa with pI of 8.7. Moreover, the protein contains a well-conserved RNA-binding domain, classifying it with a group of RNA-binding proteins with significant homology to La proteins. La proteins are conserved, predominantly nuclear phosphoproteins that bind to 3'-U termini of newly synthesized RNA polymerase III transcripts, possibly regulating mRNA stability and translation. This is the first plant clone that has a homology to La proteins. Springer-Verlag Berlin Heidelberg, 2000 Keywords Brassica napus nationallicence Microspore development nationallicence LA protein nationallicence Pollen-specific gene nationallicence Sm´ykal Petr Department of Plant Physiology, Faculty of Science, Charles University, Viniˇcná 5, 128 00 Praha, Czech Republic aut Janotová Iveta Department of Plant Physiology, Faculty of Science, Charles University, Viniˇcná 5, 128 00 Praha, Czech Republic aut Pechan P. Lehrstuhl für Pflanzenbau und Pflanzenzüchtung, Technische Universität München, 85350 Freising, Germany e-mail: 100715.331@compuserve.com Tel.: +49-8161-713421, Fax: +49-8161-714511, DE aut https://doi.org/10.1007/s004970000053 text/html Onlinezugriff via DOI 1 research-article jats NATIONALLICENCE 856 40 https://doi.org/10.1007/s004970000053 text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Sm´ykal Petr Department of Plant Physiology, Faculty of Science, Charles University, Viniˇcná 5, 128 00 Praha, Czech Republic aut NATIONALLICENCE 700 1- Janotová Iveta Department of Plant Physiology, Faculty of Science, Charles University, Viniˇcná 5, 128 00 Praha, Czech Republic aut NATIONALLICENCE 700 1- Pechan P. Lehrstuhl für Pflanzenbau und Pflanzenzüchtung, Technische Universität München, 85350 Freising, Germany e-mail: 100715.331@compuserve.com Tel.: +49-8161-713421, Fax: +49-8161-714511, DE aut Metadata rights reserved Springer special CC-BY-NC licence nationallicence BK010053 XK010053 XK010000 NATIONALLICENCE NATIONALLICENCE NL-springer