caa a22 4500 475817753 CHVBK 20180406123813.0 cr unu---uuuuu 170329e20000901xx s 000 0 eng 10.1007/s004970000044 doi (NATIONALLICENCE)springer-10.1007/s004970000044 In vitro double fertilization in Nicotiana tabacum (L.): polygamy compared with selected single pair somatic protoplast and chloroplast fusions [Elektronische Daten] [Meng-Xiang Sun, Alessandra Moscatelli, Hong-Yuan Yang, M. Cresti] Abstract : In vitro polygamy was studied mainly by using isolated sperm and central cells of tobacco in order to elucidate the mechanism that might be involved in preventing in vivo polygamy. In 17.5% 4000 M.W. polyethylene glycol, only when two sperm cells were made close enough to each other and adhered to a female cell simultaneously was polygamy possible. If one sperm cell fused with the egg or central cell, within 30 min another sperm cell could not fuse with the same egg or central cell. Similar phenomena were found in selected single somatic cell fusion. When more than two protoplasts adhered to each other simultaneously, fusion was always successful; after two protoplasts fused, within 30 min the fusion products could not fuse with another protoplast under the same conditions. This comparative study revealed this characteristic to be shared by both sexual and somatic cell fusion. However, after cytoplasm reorganization was complete in the fusion product, it was possible for the fusion product to fuse with the third protoplast. This indicates that the obstruction to additional fusion was present only during a certain period after the preceding fusion under certain condition. The possible reason for the effect is discussed. Springer-Verlag Berlin Heidelberg, 2000 Key words Cell fusion nationallicence Gamete interaction nationallicence In vitro polygamy nationallicence Nicotiana tabacum nationallicence Sun Meng-Xiang Research Center for Developmental Biology, Wuhan University, Wuhan, China, CN aut Moscatelli Alessandra Institute of Botany, Messina University, Salita Sperone 31, 98165 Messina, Italy, IT aut Yang Hong-Yuan Research Center for Developmental Biology, Wuhan University, Wuhan, China, CN aut Cresti M. Department of Environmental Science, Siena University, 53100 Siena, Italy e-mail: cresti@unisi.it Fax: +39-0577-232860, Tel.: +39-0577-232854, IT aut https://doi.org/10.1007/s004970000044 text/html Onlinezugriff via DOI 1 research-article jats NATIONALLICENCE 856 40 https://doi.org/10.1007/s004970000044 text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Sun Meng-Xiang Research Center for Developmental Biology, Wuhan University, Wuhan, China, CN aut NATIONALLICENCE 700 1- Moscatelli Alessandra Institute of Botany, Messina University, Salita Sperone 31, 98165 Messina, Italy, IT aut NATIONALLICENCE 700 1- Yang Hong-Yuan Research Center for Developmental Biology, Wuhan University, Wuhan, China, CN aut NATIONALLICENCE 700 1- Cresti M. Department of Environmental Science, Siena University, 53100 Siena, Italy e-mail: cresti@unisi.it Fax: +39-0577-232860, Tel.: +39-0577-232854, IT aut Metadata rights reserved Springer special CC-BY-NC licence nationallicence BK010053 XK010053 XK010000 NATIONALLICENCE NATIONALLICENCE NL-springer