caa a22 4500 475817761 CHVBK 20180406123813.0 cr unu---uuuuu 170329e20000901xx s 000 0 eng 10.1007/s004970000045 doi (NATIONALLICENCE)springer-10.1007/s004970000045 Minimal regions in the Arabidopsis PISTILLATA promoter responsive to the APETALA3 [Elektronische Daten] PISTILLATA feedback control do not contain a CArG box / [Xuemei Chen, José Luis Riechmann, Dongxuan Jia, Elliot Meyerowitz] Abstract : PISTILLATA (PI) is a floral homeotic B function gene in Arabidopsis and together with the other B function gene, APETALA3 (AP3), is involved in specifying petal and stamen identities. The expression of PI and AP3 is under similar developmental control. The initiation of AP3 and PI expression is at least partly caused by the floral meristem identity gene LEAFY, but the maintenance of AP3 and PI expression involves an autoregulatory loop requiring the activity of both genes. PI and AP3 are MADS domain proteins that form, and appear to function as, a heterodimer. AP3/PI binds in vitro to a sequence motif, CC(A/T)6GG, a MADS domain protein consensus binding site also known as the CArG box. We identified a 481-bp PI promoter region that confers both the initiation and the maintenance of PI expression patterns. We further dissected the promoter and identified minimal regions responsible for the AP3/PI-dependent expression. No CArG box is present in these minimal regions, suggesting that either AP3/PI does not bind directly to the PI promoter for the maintenance control, or that it requires additional factors to bind to the PI promoter. Our results suggest that the mechanisms of regulation of the two B function genes, AP3 and PI, are different, because CArG boxes are present in the AP3 promoter and are necessary for the AP3 feedback control. Springer-Verlag Berlin Heidelberg, 2000 Keywords Arabidopsis nationallicence Flower development nationallicence APETALA3 nationallicence PISTILLATA nationallicence Promoter nationallicence Chen Xuemei Division of Biology 156-29, California Institute of Technology, Pasadena, CA 91125, USA, US aut Riechmann José Luis Division of Biology 156-29, California Institute of Technology, Pasadena, CA 91125, USA, US aut Jia Dongxuan Waksman Institute, Rutgers, the State University of New Jersey, Piscataway, NJ 08854, USA e-mail: xuemei@waksman.rutgers.edu Tel.: +1-732-445-7034, Fax: +1-732-445-5735, US aut Meyerowitz Elliot Division of Biology 156-29, California Institute of Technology, Pasadena, CA 91125, USA, US aut https://doi.org/10.1007/s004970000045 text/html Onlinezugriff via DOI 1 research-article jats NATIONALLICENCE 856 40 https://doi.org/10.1007/s004970000045 text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Chen Xuemei Division of Biology 156-29, California Institute of Technology, Pasadena, CA 91125, USA, US aut NATIONALLICENCE 700 1- Riechmann José Luis Division of Biology 156-29, California Institute of Technology, Pasadena, CA 91125, USA, US aut NATIONALLICENCE 700 1- Jia Dongxuan Waksman Institute, Rutgers, the State University of New Jersey, Piscataway, NJ 08854, USA e-mail: xuemei@waksman.rutgers.edu Tel.: +1-732-445-7034, Fax: +1-732-445-5735, US aut NATIONALLICENCE 700 1- Meyerowitz Elliot Division of Biology 156-29, California Institute of Technology, Pasadena, CA 91125, USA, US aut Metadata rights reserved Springer special CC-BY-NC licence nationallicence BK010053 XK010053 XK010000 NATIONALLICENCE NATIONALLICENCE NL-springer