caa a22 4500 47581777X CHVBK 20180406123813.0 cr unu---uuuuu 170329e20000901xx s 000 0 eng 10.1007/s004970000043 doi (NATIONALLICENCE)springer-10.1007/s004970000043 Plastid differentiation during androgenesis in albino and non-albino producing cultivars of barley ( Hordeum vulgare L.) [Elektronische Daten] [S. Caredda, C. Doncoeur, P. Devaux, R. S. Sangwan, C. Clément] Abstract : In order to better understand androgenic albinism in barley, we compared plastid differentiation during anther culture in two cultivars, an albino (spring cultivar Cork) and a non-albino (winter cultivar Igri) producing cultivar. The ultrastructure of plastids and the relative amount of DNA containing plastids were followed in both cultivars during the androgenic process and correlated with the proportion of regenerated chlorophyllous plantlets. For androgenesis, anthers were collected at the uninucleate stage, during mid- or late-microspore vacuolation. At this stage DNA was detected in 15.3 ± 2. 7% of microspore plastid sections in the winter cultivar Igri, compared to 1.7 ± 0.5% in the spring cultivar Cork. In the winter cultivar Igri, starch was broken down after anther pretreatment but plastids divided rapidly during anther culture and thylakoids developed in the stroma. Prior to regeneration, plastids contained 2.0 ± 0.2 thylakoids per plastid and starch represented 26.1 ± 3.3% of the plastid volume. In the spring cultivar Cork, plastids followed a different developmental pathway. After anther pretreatment, microspore plastids differentiated exclusively into amyloplasts, accumulating starch and losing their thylakoids as well as their capacity to divide. This developmental pattern became progressively more marked, so that by the end of anther culture plastids contained 0.5 ± 0.4 thylakoids per plastid and starch represented up to 90.3 ± 4.3% of plastid volume. Following androgenesis, the response was similar in both cultivars except that the winter cultivar Igri provided 87.8% of chlorophyllous plantlets compared to 99.7% albino plantlets in the cultivar Cork. The results presented here suggest that the exclusive regeneration of albino plantlets in the spring cultivar Cork may be due to degradation of microspore plastid DNA during early pollen development, preventing the plastids from differentiating into chloroplasts under culture conditions. Springer-Verlag Berlin Heidelberg, 2000 Key words Androgenesis nationallicence Albinism nationallicence Hordeum vulgare L nationallicence Plastid differentiation nationallicence Plastid DNA nationallicence Pollen development nationallicence Caredda S. Université de Reims Champagne Ardenne, UFR Sciences, Biologie et Physiologie Végétales, BP 1039; 51687 Reims Cédex 2, France E-mail: christophe.clement@univ.reims.fr, FR aut Doncoeur C. Université de Reims Champagne Ardenne, UFR Sciences, Biologie et Physiologie Végétales, BP 1039; 51687 Reims Cédex 2, France E-mail: christophe.clement@univ.reims.fr, FR aut Devaux P. Florimond Desprez Ind., Section Biotechnologies, BP 41; 59242 Cappelle en Pévèle, France, FR aut Sangwan R. S. Université de Picardie Jules Verne, Androgénèse et Biotechnologies, 33, rue Saint-Leu; 80039 Amiens, France, FR aut Clément C. Université de Reims Champagne Ardenne, UFR Sciences, Biologie et Physiologie Végétales, BP 1039; 51687 Reims Cédex 2, France E-mail: christophe.clement@univ.reims.fr, FR aut https://doi.org/10.1007/s004970000043 text/html Onlinezugriff via DOI 1 research-article jats NATIONALLICENCE 856 40 https://doi.org/10.1007/s004970000043 text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Caredda S. Université de Reims Champagne Ardenne, UFR Sciences, Biologie et Physiologie Végétales, BP 1039; 51687 Reims Cédex 2, France E-mail: christophe.clement@univ.reims.fr, FR aut NATIONALLICENCE 700 1- Doncoeur C. Université de Reims Champagne Ardenne, UFR Sciences, Biologie et Physiologie Végétales, BP 1039; 51687 Reims Cédex 2, France E-mail: christophe.clement@univ.reims.fr, FR aut NATIONALLICENCE 700 1- Devaux P. Florimond Desprez Ind., Section Biotechnologies, BP 41; 59242 Cappelle en Pévèle, France, FR aut NATIONALLICENCE 700 1- Sangwan R. S. Université de Picardie Jules Verne, Androgénèse et Biotechnologies, 33, rue Saint-Leu; 80039 Amiens, France, FR aut NATIONALLICENCE 700 1- Clément C. Université de Reims Champagne Ardenne, UFR Sciences, Biologie et Physiologie Végétales, BP 1039; 51687 Reims Cédex 2, France E-mail: christophe.clement@univ.reims.fr, FR aut Metadata rights reserved Springer special CC-BY-NC licence nationallicence BK010053 XK010053 XK010000 NATIONALLICENCE NATIONALLICENCE NL-springer