caa a22 4500 475818245 CHVBK 20180406123815.0 cr unu---uuuuu 170329e20000401xx s 000 0 eng 10.1007/s002280050717 doi (NATIONALLICENCE)springer-10.1007/s002280050717 Nilvadipine protects low-density lipoprotein cholesterol from in vivo oxidation in hypertensive patients with risk factors for atherosclerosis [Elektronische Daten] [M. Inouye, T. Mio, K. Sumino] Objective: Nilvadipine, a calcium antagonist, has been shown to have antioxidant activity in vitro, but its effect on in vivo oxidation has not been assessed. The aim of this study was to investigate the antioxidant effect of this agent in vivo. The ratios of 7-keto cholestadien to cholesterol are believed to be an available marker of lipid peroxidation. Using these ratios, we have assessed the antioxidant effect of nilvadipine on low-density lipoprotein (LDL) in hypertensive patients with high risk of atherosclerosis. The risk factors of atherosclerosis may involve oxidation of LDL. Methods: Fifteen healthy subjects (seven females and eight males aged 35-72 years, mean ± SD=55.3 ± 13.8 years) and fifteen hypertensive patients (seven females and eight males aged 45-80 years, mean ± SD = 60.2 ± 11.8 years) were recruited. Patients were treated orally with nilvadipine (4 mg b.i.d.) for 4 weeks. Cholesterol oxidation levels of LDL in patients before and after nilvadipine therapy and healthy subjects were studied. Results: The ratios of 7-keto cholestadien to cholesterol in LDL of hypertensive patients before and 4 weeks after nilvadipine treatment and in healthy subjects were 6.5 ± 1.6% (mean ± SD), 3.8 ± 1.2%, and 0.2 ± 0.1%, respectively. There were significantly (P < 0.001) increased levels of cholesterol oxidation in LDL in patients with hypertension both before and after nilvadipine treatment compared with healthy subjects. However, there was a significantly (P < 0.001) decreased level of cholesterol oxidation in LDL in patients after nilvadipine treatment compared with patients before nilvadipine treatment. Conclusion: Our data showed that nilvadipine may protect LDL cholesterol from in vivo oxidation in hypertensive patients with high risk of atherosclerosis. Springer-Verlag Berlin Heidelberg, 2000 Key words Nilvadipine nationallicence Hypertension nationallicence Atherosclerosis nationallicence Inouye M. Department of Internal Medicine, Hyogo Rehabilitation Center Hospital, Akebono-cho 1070, Nishi-ku, Kobe 651-2181, Japan Tel.: +81-78-9272727; Fax: +81-78-9259203, JP aut Mio T. Department of Public Health, Kobe University School of Medicine, 7-chome, Kusunoki-cho, Chuo-ku, Kobe 650-0017, Japan, JP aut Sumino K. Department of Public Health, Kobe University School of Medicine, 7-chome, Kusunoki-cho, Chuo-ku, Kobe 650-0017, Japan, JP aut https://doi.org/10.1007/s002280050717 text/html Onlinezugriff via DOI 1 research-article jats NATIONALLICENCE 856 40 https://doi.org/10.1007/s002280050717 text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Inouye M. Department of Internal Medicine, Hyogo Rehabilitation Center Hospital, Akebono-cho 1070, Nishi-ku, Kobe 651-2181, Japan Tel.: +81-78-9272727; Fax: +81-78-9259203, JP aut NATIONALLICENCE 700 1- Mio T. Department of Public Health, Kobe University School of Medicine, 7-chome, Kusunoki-cho, Chuo-ku, Kobe 650-0017, Japan, JP aut NATIONALLICENCE 700 1- Sumino K. Department of Public Health, Kobe University School of Medicine, 7-chome, Kusunoki-cho, Chuo-ku, Kobe 650-0017, Japan, JP aut Metadata rights reserved Springer special CC-BY-NC licence nationallicence BK010053 XK010053 XK010000 NATIONALLICENCE NATIONALLICENCE NL-springer