caa a22 4500 475818989 CHVBK 20180406123817.0 cr unu---uuuuu 170329e20000801xx s 000 0 eng 10.1007/s002280000143 doi (NATIONALLICENCE)springer-10.1007/s002280000143 Metabolism of chloramphenicol succinate in human bone marrow [Elektronische Daten] [C. S. Ambekar, B. Cheung, J. Lee, L. C. Chan, R. Liang, C. R. Kumana] Objective/methods: The metabolism of chloramphenicol succinate (CAPS) by human bone marrow was studied in vitro using 75 marrow samples. Whole marrow samples were incubated with CAPS with or without reduced nicotinamide adenine dinucleotide phosphate for 1, 2 and 3 h at 37 °C. Ficoll-paque-separated marrow mononuclear cells and erythrocytes were similarly incubated. After precipitation and centrifugation, clear supernatant was analysed for the presence of metabolites using high-performance liquid chromatography Results: Only one metabolite was detected when CAPS was incubated for 3 h with whole marrow from 72 donors. Its retention time (RT 10.9 min) corresponded to chloramphenicol (CAP). When CAPS was incubated with samples of whole marrow, marrow mononuclear cells, marrow erythrocytes, marrow plasma and peripheral blood from one donor who had taken Traditional Chinese Medicine (TCM), three metabolite peaks were detected within 15 min to 1 h. The RT of two of these peaks corresponded to CAP and nitroso-CAP (RT 14.9 min), but one peak remained unidentified. These peaks were not detected in the control samples incubated without CAPS. Blood samples collected after 3 months and 6 months to reconfirm metabolic activity yielded no such metabolite peaks when incubated with CAPS for 1-3 h. Therefore induction of enzyme activity by TCM was suspected. Three metabolite peaks with the same RTs were also detected when CAPS was incubated for 3 h with whole marrow from two other donors. Conclusion: These studies demonstrated that CAPS may be metabolised to CAP and occasionally other metabolites in human bone marrow. This novel observation is particularly important because the bone marrow is known to be a target organ for chloramphenicol toxicity. Springer-Verlag Berlin Heidelberg, 2000 Key words Chloramphenicol succinate nationallicence Chloramphenicol nationallicence Human bone marrow nationallicence Ambekar C. S. Departments of Medicine, Pathology and the Clinical Biochemistry Unit, The University of Hong Kong, Queen Mary Hospital, 102 Pokfulam Rd, Hong Kong e-mail: ambekar@hknet.com Tel.: +852-256-68983, CN aut Cheung B. Departments of Medicine, Pathology and the Clinical Biochemistry Unit, The University of Hong Kong, Queen Mary Hospital, 102 Pokfulam Rd, Hong Kong e-mail: ambekar@hknet.com Tel.: +852-256-68983, CN aut Lee J. Departments of Medicine, Pathology and the Clinical Biochemistry Unit, The University of Hong Kong, Queen Mary Hospital, 102 Pokfulam Rd, Hong Kong e-mail: ambekar@hknet.com Tel.: +852-256-68983, CN aut Chan L. C. Departments of Medicine, Pathology and the Clinical Biochemistry Unit, The University of Hong Kong, Queen Mary Hospital, 102 Pokfulam Rd, Hong Kong e-mail: ambekar@hknet.com Tel.: +852-256-68983, CN aut Liang R. Departments of Medicine, Pathology and the Clinical Biochemistry Unit, The University of Hong Kong, Queen Mary Hospital, 102 Pokfulam Rd, Hong Kong e-mail: ambekar@hknet.com Tel.: +852-256-68983, CN aut Kumana C. R. Departments of Medicine, Pathology and the Clinical Biochemistry Unit, The University of Hong Kong, Queen Mary Hospital, 102 Pokfulam Rd, Hong Kong e-mail: ambekar@hknet.com Tel.: +852-256-68983, CN aut https://doi.org/10.1007/s002280000143 text/html Onlinezugriff via DOI 1 research-article jats NATIONALLICENCE 856 40 https://doi.org/10.1007/s002280000143 text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Ambekar C. S. Departments of Medicine, Pathology and the Clinical Biochemistry Unit, The University of Hong Kong, Queen Mary Hospital, 102 Pokfulam Rd, Hong Kong e-mail: ambekar@hknet.com Tel.: +852-256-68983, CN aut NATIONALLICENCE 700 1- Cheung B. Departments of Medicine, Pathology and the Clinical Biochemistry Unit, The University of Hong Kong, Queen Mary Hospital, 102 Pokfulam Rd, Hong Kong e-mail: ambekar@hknet.com Tel.: +852-256-68983, CN aut NATIONALLICENCE 700 1- Lee J. Departments of Medicine, Pathology and the Clinical Biochemistry Unit, The University of Hong Kong, Queen Mary Hospital, 102 Pokfulam Rd, Hong Kong e-mail: ambekar@hknet.com Tel.: +852-256-68983, CN aut NATIONALLICENCE 700 1- Chan L. C. Departments of Medicine, Pathology and the Clinical Biochemistry Unit, The University of Hong Kong, Queen Mary Hospital, 102 Pokfulam Rd, Hong Kong e-mail: ambekar@hknet.com Tel.: +852-256-68983, CN aut NATIONALLICENCE 700 1- Liang R. Departments of Medicine, Pathology and the Clinical Biochemistry Unit, The University of Hong Kong, Queen Mary Hospital, 102 Pokfulam Rd, Hong Kong e-mail: ambekar@hknet.com Tel.: +852-256-68983, CN aut NATIONALLICENCE 700 1- Kumana C. R. Departments of Medicine, Pathology and the Clinical Biochemistry Unit, The University of Hong Kong, Queen Mary Hospital, 102 Pokfulam Rd, Hong Kong e-mail: ambekar@hknet.com Tel.: +852-256-68983, CN aut Metadata rights reserved Springer special CC-BY-NC licence nationallicence BK010053 XK010053 XK010000 NATIONALLICENCE NATIONALLICENCE NL-springer