caa a22 4500 475819144 CHVBK 20180406123817.0 cr unu---uuuuu 170329e20001201xx s 000 0 eng 10.1007/s002280000203 doi (NATIONALLICENCE)springer-10.1007/s002280000203 Correlation between acetylation phenotype and genotype in Chinese women [Elektronische Daten] [Bin Zhao, Adeline Seow, Edmund J.D. Lee, Hin-Peng Lee] Abstract.: Objective: The acetylation polymorphism is a common inherited variation in human drug and carcinogen metabolism. Because N-acetyltransferase (NAT2) is important for the detoxification and/or bioactivation of drugs and carcinogens, this polymorphism has important implications in therapeutics and cancer susceptibility. A high correlation between acetylation phenotype and genotype has been demonstrated in several studies. However, no such data exist for Chinese females. The aim of the present study was to compare acetylation phenotype with NAT2 genotype in a population of primarily non-smoking Chinese females. Methods: In the present study, the correlation between N-acetyltransferase activity and NAT2 genotype was evaluated in 103 unrelated Chinese female controls derived from a hospital-based case-control study of lung cancer in Singapore. Acetylation phenotype and genotype were respectively determined using caffeine and an allele-specific polymerase chain reaction (PCR). Results: The proportions of rapid and slow phenotypes were 78% and 22%, respectively, while the distribution of rapid (heterozygotes and homozygotes combined) and slow acetylator genotypes was 76% and 24%, respectively. The distribution of the various NAT2 genotypes did not differ significantly (X 2=1.45, P>0.05) from that predicted by the Hardy-Weinberg Law. All slow acetylators were accurately predicted (100%), whereas 2 of 80 rapid acetylators were erroneously predicted as slow (2.5%). The overall prediction rate of the PCR-based test for the acetylation phenotype was at 98.1% in our Chinese population. Conclusion: Our results suggest that genotyping with PCR may well become the preferred method for the determination of acetylation polymorphism in epidemiological studies in this Asian population. Springer-Verlag, 2000 N-acetyltransferase Chinese women Caffeine metabolites nationallicence Zhao Bin Department of Community, Occupational and Family Medicine, Faculty of Medicine, National University of Singapore, 16 Medical Drive, Singapore 117597, Republic of Singapore aut Seow Adeline Department of Community, Occupational and Family Medicine, Faculty of Medicine, National University of Singapore, 16 Medical Drive, Singapore 117597, Republic of Singapore aut Lee Edmund J.D. Department of Pharmacology, Faculty of Medicine, Lower Kent Ridge Road, National University of Singapore, Singapore 119260, Republic of Singapore aut Lee Hin-Peng Department of Community, Occupational and Family Medicine, Faculty of Medicine, National University of Singapore, 16 Medical Drive, Singapore 117597, Republic of Singapore aut European Journal of Clinical Pharmacology Springer-Verlag 56/9-10(2000-12-01), 689-692 0031-6970 56:9-10<689 2000 56 228 https://doi.org/10.1007/s002280000203 text/html Onlinezugriff via DOI 1 research-article jats NATIONALLICENCE 856 40 https://doi.org/10.1007/s002280000203 text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Zhao Bin Department of Community, Occupational and Family Medicine, Faculty of Medicine, National University of Singapore, 16 Medical Drive, Singapore 117597, Republic of Singapore aut NATIONALLICENCE 700 1- Seow Adeline Department of Community, Occupational and Family Medicine, Faculty of Medicine, National University of Singapore, 16 Medical Drive, Singapore 117597, Republic of Singapore aut NATIONALLICENCE 700 1- Lee Edmund J.D. Department of Pharmacology, Faculty of Medicine, Lower Kent Ridge Road, National University of Singapore, Singapore 119260, Republic of Singapore aut NATIONALLICENCE 700 1- Lee Hin-Peng Department of Community, Occupational and Family Medicine, Faculty of Medicine, National University of Singapore, 16 Medical Drive, Singapore 117597, Republic of Singapore aut NATIONALLICENCE 773 0- European Journal of Clinical Pharmacology Springer-Verlag 56/9-10(2000-12-01), 689-692 0031-6970 56:9-10<689 2000 56 228 Metadata rights reserved Springer special CC-BY-NC licence nationallicence BK010053 XK010053 XK010000 NATIONALLICENCE NATIONALLICENCE NL-springer