caa a22 4500 475824393 CHVBK 20180406123827.0 cr unu---uuuuu 170329e20000801xx s 000 0 eng 10.1023/A:1018738502654 doi (NATIONALLICENCE)springer-10.1023/A:1018738502654 Soluble P-selectin Is Not a Surrogate Marker for Platelet P-selectin: Evidence from a Multicenter Chest Pain Study Group [Elektronische Daten] [Paul Gurbel, Dean Kereiakes, Victor Serebruany] It has been reported that platelet expression and plasma levels of soluble P-selectin are increased in patients with unstable coronary artery syndromes. However, the origin of soluble P-selectin remains unknown. We sought to determine whether platelet expression of P-selectin correlates with plasma levels in the population of patients presenting to the emergency department with chest pain. In 338 patients presenting with chest pain to the emergency departments of three different hospitals, simultaneous soluble and platelet P-selection levels were determined using enzyme-linked immunosorbent assay (ELISA) and whole blood flow cytometry, respectively. Using regression analysis no correlation (R2=0.055) was found between soluble and platelet-bound P-selectin for the study population, including those patients with noncardiac chest pain (R2=0.019), unstable angina (R2=0.007), acute myocardial infraction (R2=0.033), congestive heart failure (R2=0.231), and gastrointestinal illness (R2=0.020). The platelet expression of P-selectin is unrelated to the level found in plasma in patients with acute chest pain, irrespective of the etiology of chest pain. Dissociation between platelet and soluble P-selectin suggests that the soluble form cannot serve as a surrogate marker to indicate platelet activation in the chest pain population. Kluwer Academic Publishers, 2000 P-selectin nationallicence flow cytometry nationallicence ELISA nationallicence chest pain nationallicence emergency medicine nationallicence Gurbel Paul Sinai Center for Thrombosis Research, Baltimore, Maryland aut Kereiakes Dean Lindner Research Center, Cincinnati, Ohio, USA aut Serebruany Victor Sinai Center for Thrombosis Research, Baltimore, Maryland aut Journal of Thrombosis and Thrombolysis Kluwer Academic Publishers 10/1(2000-08-01), 15-22 0929-5305 10:1<15 2000 10 11239 https://doi.org/10.1023/A:1018738502654 text/html Onlinezugriff via DOI 1 research-article jats NATIONALLICENCE 856 40 https://doi.org/10.1023/A:1018738502654 text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Gurbel Paul Sinai Center for Thrombosis Research, Baltimore, Maryland aut NATIONALLICENCE 700 1- Kereiakes Dean Lindner Research Center, Cincinnati, Ohio, USA aut NATIONALLICENCE 700 1- Serebruany Victor Sinai Center for Thrombosis Research, Baltimore, Maryland aut NATIONALLICENCE 773 0- Journal of Thrombosis and Thrombolysis Kluwer Academic Publishers 10/1(2000-08-01), 15-22 0929-5305 10:1<15 2000 10 11239 Metadata rights reserved Springer special CC-BY-NC licence nationallicence BK010053 XK010053 XK010000 NATIONALLICENCE NATIONALLICENCE NL-springer