caa a22 4500 475824733 CHVBK 20180406123828.0 cr unu---uuuuu 170329e20001001xx s 000 0 eng 10.1023/A:1018770711705 doi (NATIONALLICENCE)springer-10.1023/A:1018770711705 What is the Required Reperfusion Period for Assessment of Myocardial Infarct Size Using Triphenyltetrazolium Chloride Staining in the Rat? [Elektronische Daten] [Ernst Schwarz, Yvette Somoano, Sharon Hale, Robert Kloner] Measurements of infarct size by use of tripenyltetrazoliumchloride (TTC) is a widely accepted method used to delineate the extent of myocardial necrosis following coronary occlusion and reperfusion in various animal experiments. There is controversy, however, regarding the optimal reperfusion time for estimating the maximally infarcted area by TTC staining in the rat. We tested six different reperfusion times following 90 minutes of regional myocardial ischemia. Group 1 had 5 minutes of reperfusion (n=6), group 2 had 30 minutes of reperfusion (n=6), group 3 had 1 hour of reperfusion (n=6), group 4 had 2 hours of reperfusion (n=6), group 5 had 3 hours of reperfusion (n=6), group 6 had 4.5 hours of reperfusion (n=6). Risk areas, measured by the use of blue dye, were similar among the 6 study groups. Infarct size as a percent of risk area was 57±11% in group 1, 74±7% in group 2, 61±9% in group 3, 71±5% in group 4, 70±5% in group 5, and 64±9% in group 6 (x±standard error [SE]). There was no signifcant difference in infarct size between the groups. However, prior to 60 minutes of reperfusion, patches of pink and white areas were observed within the risk regions, indicating a more difficult assessment of proper delineation of outer regions of necrotic tissue. For acute assessment of infarct size, reperfusion for 60 minutes or more is optimal because the infarct does appear homogeneous at that time and does not become larger with longer reperfusion periods. Kluwer Academic Publishers, 2000 infarct size nationallicence triphenyltetrazolium nationallicence reperfusion nationallicence rats nationallicence Schwarz Ernst Heart Institute, Good Samaritan Hospital & Division of Cardiology, University of Southern California, Los Angeles, California, USA aut Somoano Yvette Heart Institute, Good Samaritan Hospital & Division of Cardiology, University of Southern California, Los Angeles, California, USA aut Hale Sharon Heart Institute, Good Samaritan Hospital & Division of Cardiology, University of Southern California, Los Angeles, California, USA aut Kloner Robert Heart Institute, Good Samaritan Hospital & Division of Cardiology, University of Southern California, Los Angeles, California, USA aut Journal of Thrombosis and Thrombolysis Kluwer Academic Publishers 10/2(2000-10-01), 181-187 0929-5305 10:2<181 2000 10 11239 https://doi.org/10.1023/A:1018770711705 text/html Onlinezugriff via DOI 1 research-article jats NATIONALLICENCE 856 40 https://doi.org/10.1023/A:1018770711705 text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Schwarz Ernst Heart Institute, Good Samaritan Hospital & Division of Cardiology, University of Southern California, Los Angeles, California, USA aut NATIONALLICENCE 700 1- Somoano Yvette Heart Institute, Good Samaritan Hospital & Division of Cardiology, University of Southern California, Los Angeles, California, USA aut NATIONALLICENCE 700 1- Hale Sharon Heart Institute, Good Samaritan Hospital & Division of Cardiology, University of Southern California, Los Angeles, California, USA aut NATIONALLICENCE 700 1- Kloner Robert Heart Institute, Good Samaritan Hospital & Division of Cardiology, University of Southern California, Los Angeles, California, USA aut NATIONALLICENCE 773 0- Journal of Thrombosis and Thrombolysis Kluwer Academic Publishers 10/2(2000-10-01), 181-187 0929-5305 10:2<181 2000 10 11239 Metadata rights reserved Springer special CC-BY-NC licence nationallicence BK010053 XK010053 XK010000 NATIONALLICENCE NATIONALLICENCE NL-springer