caa a22 4500 475830946 CHVBK 20180406123839.0 cr unu---uuuuu 170329e20000201xx s 000 0 eng 10.1007/s100960050009 doi (NATIONALLICENCE)springer-10.1007/s100960050009 Limits of Detection of Mycobacterium tuberculosis in Spiked Cerebrospinal Fluid Using the Polymerase Chain Reaction in Tuberculous Meningitis [Elektronische Daten] [J. Baran Jr., K. M. Riederer, R. Khatib]  The limit of detection of Mycobacterium tuberculosis in spiked cerebrospinal fluid (CSF) using polymerase chain reaction (PCR) was compared to that of a radiometric liquid culture. Serial dilutions of clinical isolates of Mycobacterium tuberculosis were prepared in CSF (n=3) or broth (n=11) with estimated concentrations of 0-550 cfu/ml. Each dilution was examined concurrently by PCR and radiometric culture. PCR and radiometric culture detected Mycobacterium tuberculosis DNA in all dilutions with an estimated 2 cfu/ml in the CSF. At lower concentrations (estimated <2 cfu/ml), PCR and radiometric culture were positive in three of five (60%) and five of five (100%) CSF samples, respectively. In comparison to PCR in broth dilutions, no evidence of inhibition or interference was noted. These results imply that PCR can provide a rapid and reliable diagnosis of tuberculous meningitis, although there is a potential for false-negative results to occur in samples containing very few organisms (<2 cfu/ml). Springer-Verlag Berlin Heidelberg, 2000 Baran Jr. J. Medical Education Department, St. John Hospital and Medical Center, 22101 Moross Road, Detroit, MI 48236, USA, US aut Riederer K. M. Department of Medicine, Division of Infectious Diseases, St. John Hospital and Medical Center, 22101 Moross Road, Detroit, MI 48236, USA, US aut Khatib R. Department of Medicine, Division of Infectious Diseases, St. John Hospital and Medical Center, 22101 Moross Road, Detroit, MI 48236, USA, US aut https://doi.org/10.1007/s100960050009 text/html Onlinezugriff via DOI 1 research-article jats NATIONALLICENCE 856 40 https://doi.org/10.1007/s100960050009 text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Baran Jr J. Medical Education Department, St. John Hospital and Medical Center, 22101 Moross Road, Detroit, MI 48236, USA, US aut NATIONALLICENCE 700 1- Riederer K. M. Department of Medicine, Division of Infectious Diseases, St. John Hospital and Medical Center, 22101 Moross Road, Detroit, MI 48236, USA, US aut NATIONALLICENCE 700 1- Khatib R. Department of Medicine, Division of Infectious Diseases, St. John Hospital and Medical Center, 22101 Moross Road, Detroit, MI 48236, USA, US aut Metadata rights reserved Springer special CC-BY-NC licence nationallicence BK010053 XK010053 XK010000 NATIONALLICENCE NATIONALLICENCE NL-springer