caa a22 4500 475831217 CHVBK 20180406123840.0 cr unu---uuuuu 170329e20000701xx s 000 0 eng 10.1007/s100960000290 doi (NATIONALLICENCE)springer-10.1007/s100960000290 Novel Genus-Specific PCR-Based Assays for Rapid Identification of Neisseria Species and Neisseria meningitidis [Elektronische Daten] [N. Lansac, F. J. Picard, C. Ménard, M. Boissinot, M. Ouellette, P. H. Roy, M. G. Bergeron]  This study presents the development ofpolymerase chain reaction (PCR)-based tests for the identification and detection of Neisseria species and Neisseria meningitidis. Currently, isolating and identifying these pathogens using conventional biochemical methods require 48-72 h. To improve speed and accuracy in diagnosing Neisseria infections, simple PCR-based tests that are specific for the genus Neisseria and the species Neisseria meningitidis have been developed. The genus-specific and species-specific DNA sequences were chosen by selecting and analyzing available database sequences. Neisseria-specific and Neisseria meningitidis-specific primer pairs were derived from the genes asd (coding for the aspartate β-semialdehyde dehydrogenase) and ctrA (coding for a conserved outer membrane protein), respectively. Both the Neisseria-specific and Neisseria meningitidis-specific PCR assays were specific (they amplified only DNA from the target genus or species, out of 84 bacterial species tested). In addition, the Neisseria-specific assay amplified DNA from 321 of 322 strains tested representing 13 species of Neisseria, while the Neisseria meningitidis-specific assay amplified DNA from all 256 strains tested representing nine serogroups of Neisseria meningitidis. These PCR assays, which can be combined in multiplex, have been adapted to ensure that they are simple and can be performed within approximately 90 min. The tests provide new diagnostic tools for identifying Neisseria infections. Springer-Verlag Berlin Heidelberg, 2000 Lansac N. Division de Microbiologie, Faculté de Médecine, Université Laval, Quebec, Canada, G1V 4G2., CA aut Picard F. J. Centre de Recherche en Infectiologie, CHUQ (Pavillon CHUL), 2705 Boulevard Laurier, Ste-Foy, Quebec, Canada, G1V 4G2 e-mail: Michel.G.Bergeron@crchul.ulaval.ca, CA aut Ménard C. Centre de Recherche en Infectiologie, CHUQ (Pavillon CHUL), 2705 Boulevard Laurier, Ste-Foy, Quebec, Canada, G1V 4G2 e-mail: Michel.G.Bergeron@crchul.ulaval.ca, CA aut Boissinot M. Division de Microbiologie, Faculté de Médecine, Université Laval, Quebec, Canada, G1V 4G2., CA aut Ouellette M. Division de Microbiologie, Faculté de Médecine, Université Laval, Quebec, Canada, G1V 4G2., CA aut Roy P. H. Département de Biochimie, Université Laval, Quebec, Canada, G1K 7P4, CA aut Bergeron M. G. Division de Microbiologie, Faculté de Médecine, Université Laval, Quebec, Canada, G1V 4G2., CA aut https://doi.org/10.1007/s100960000290 text/html Onlinezugriff via DOI 1 research-article jats NATIONALLICENCE 856 40 https://doi.org/10.1007/s100960000290 text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Lansac N. Division de Microbiologie, Faculté de Médecine, Université Laval, Quebec, Canada, G1V 4G2., CA aut NATIONALLICENCE 700 1- Picard F. J. Centre de Recherche en Infectiologie, CHUQ (Pavillon CHUL), 2705 Boulevard Laurier, Ste-Foy, Quebec, Canada, G1V 4G2 e-mail: Michel.G.Bergeron@crchul.ulaval.ca, CA aut NATIONALLICENCE 700 1- Ménard C. Centre de Recherche en Infectiologie, CHUQ (Pavillon CHUL), 2705 Boulevard Laurier, Ste-Foy, Quebec, Canada, G1V 4G2 e-mail: Michel.G.Bergeron@crchul.ulaval.ca, CA aut NATIONALLICENCE 700 1- Boissinot M. Division de Microbiologie, Faculté de Médecine, Université Laval, Quebec, Canada, G1V 4G2., CA aut NATIONALLICENCE 700 1- Ouellette M. Division de Microbiologie, Faculté de Médecine, Université Laval, Quebec, Canada, G1V 4G2., CA aut NATIONALLICENCE 700 1- Roy P. H. Département de Biochimie, Université Laval, Quebec, Canada, G1K 7P4, CA aut NATIONALLICENCE 700 1- Bergeron M. G. Division de Microbiologie, Faculté de Médecine, Université Laval, Quebec, Canada, G1V 4G2., CA aut Metadata rights reserved Springer special CC-BY-NC licence nationallicence BK010053 XK010053 XK010000 NATIONALLICENCE NATIONALLICENCE NL-springer