caa a22 4500 47583125X CHVBK 20180406123840.0 cr unu---uuuuu 170329e20000701xx s 000 0 eng 10.1007/s100960000297 doi (NATIONALLICENCE)springer-10.1007/s100960000297 Usefulness of Simultaneous Detection of Toxin A and Glutamate Dehydrogenase for the Diagnosis of Clostridium difficile -Associated Diseases [Elektronische Daten] [F. Barbut, V. Lalande, G. Daprey, P. Cohen, N. Marle, B. Burghoffer, J. C. Petit]  The aim of this study was to evaluate an immunoassay (Triage; Biosite Diagnostics, BMD, France) for detecting both a specific antigen of Clostridium difficile (glutamate dehydrogenase [GDH]) and toxin A. Evaluation of the test was carried out in 304 fecal samples from patients suspected of having Clostridium difficile-associated diseases. The results with GDH and toxin A were compared with those of a culture and cytotoxicity assay (toxin B). The prevalence rates for toxin B-positive and culture-positive fecal samples were 11.2% and 25%, respectively. The sensitivity of the Triage immunoassay was 90.8% for GDH and 79.4% for toxin A. A negative result with both toxin A and GDH was very reliably able to eliminate a diagnosis of Clostridium difficile-associated disease (negative predictive value 99.6%). Triage is a very rapid (20 min) and easy-to-perform test. It could be useful for diagnostic purposes and also for detecting nontoxigenic strains in epidemiogical studies. Springer-Verlag Berlin Heidelberg, 2000 Barbut F. Unité d'Hygiène et de Lutte contre les Infections Nosocomiales (UHLIN), Hôpital Saint-Antoine, Assistance-Publique-Hôpitaux de Paris, 184 rue du Faubourg Saint-Antoine, 75012 Paris, France e-mail: frederic.barbut@sat.ap-hop-paris.fr, FR aut Lalande V. Service de Bactériologie-Virologie, Hôpital Saint-Antoine, Assistance-Publique-Hôpitaux de Paris, 184 rue du Faubourg Saint-Antoine, 75012 Paris, France, FR aut Daprey G. Service de Bactériologie-Virologie, Hôpital Saint-Antoine, Assistance-Publique-Hôpitaux de Paris, 184 rue du Faubourg Saint-Antoine, 75012 Paris, France, FR aut Cohen P. Service de Bactériologie-Virologie, Hôpital Saint-Antoine, Assistance-Publique-Hôpitaux de Paris, 184 rue du Faubourg Saint-Antoine, 75012 Paris, France, FR aut Marle N. Service de Bactériologie-Virologie, Hôpital Saint-Antoine, Assistance-Publique-Hôpitaux de Paris, 184 rue du Faubourg Saint-Antoine, 75012 Paris, France, FR aut Burghoffer B. Service de Bactériologie-Virologie, Hôpital Saint-Antoine, Assistance-Publique-Hôpitaux de Paris, 184 rue du Faubourg Saint-Antoine, 75012 Paris, France, FR aut Petit J. C. Service de Bactériologie-Virologie, Hôpital Saint-Antoine, Assistance-Publique-Hôpitaux de Paris, 184 rue du Faubourg Saint-Antoine, 75012 Paris, France, FR aut https://doi.org/10.1007/s100960000297 text/html Onlinezugriff via DOI 1 research-article jats NATIONALLICENCE 856 40 https://doi.org/10.1007/s100960000297 text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Barbut F. Unité d'Hygiène et de Lutte contre les Infections Nosocomiales (UHLIN), Hôpital Saint-Antoine, Assistance-Publique-Hôpitaux de Paris, 184 rue du Faubourg Saint-Antoine, 75012 Paris, France e-mail: frederic.barbut@sat.ap-hop-paris.fr, FR aut NATIONALLICENCE 700 1- Lalande V. Service de Bactériologie-Virologie, Hôpital Saint-Antoine, Assistance-Publique-Hôpitaux de Paris, 184 rue du Faubourg Saint-Antoine, 75012 Paris, France, FR aut NATIONALLICENCE 700 1- Daprey G. Service de Bactériologie-Virologie, Hôpital Saint-Antoine, Assistance-Publique-Hôpitaux de Paris, 184 rue du Faubourg Saint-Antoine, 75012 Paris, France, FR aut NATIONALLICENCE 700 1- Cohen P. Service de Bactériologie-Virologie, Hôpital Saint-Antoine, Assistance-Publique-Hôpitaux de Paris, 184 rue du Faubourg Saint-Antoine, 75012 Paris, France, FR aut NATIONALLICENCE 700 1- Marle N. Service de Bactériologie-Virologie, Hôpital Saint-Antoine, Assistance-Publique-Hôpitaux de Paris, 184 rue du Faubourg Saint-Antoine, 75012 Paris, France, FR aut NATIONALLICENCE 700 1- Burghoffer B. Service de Bactériologie-Virologie, Hôpital Saint-Antoine, Assistance-Publique-Hôpitaux de Paris, 184 rue du Faubourg Saint-Antoine, 75012 Paris, France, FR aut NATIONALLICENCE 700 1- Petit J. C. Service de Bactériologie-Virologie, Hôpital Saint-Antoine, Assistance-Publique-Hôpitaux de Paris, 184 rue du Faubourg Saint-Antoine, 75012 Paris, France, FR aut Metadata rights reserved Springer special CC-BY-NC licence nationallicence BK010053 XK010053 XK010000 NATIONALLICENCE NATIONALLICENCE NL-springer