caa a22 4500 475831284 CHVBK 20180406123840.0 cr unu---uuuuu 170329e20000701xx s 000 0 eng 10.1007/s100960000285 doi (NATIONALLICENCE)springer-10.1007/s100960000285 Use of the Ligase Detection Reaction-Polymerase Chain Reaction to Identify Point Mutations in Extended-Spectrum Beta-Lactamases [Elektronische Daten] [C. Niederhauser, L. Kaempf, I. Heinzer]  The aim of this study was to detect point mutations in extended-spectrum β-lactamase (ESBL) genes in a background of wild-type (non-ESBL-producing) bacteria using a highly sensitive and specific method developed for this purpose. The ligase detection reaction-polymerase chain reaction (LDR-PCR) method was used to test different ESBL-producing strains and clinical isolates for a specific point mutation in the bla SHV-ESBL gene (glycine to serine mutation at position 238) and was compared with the commercially available E test ESBL (AB Biodisk, Sweden). Nine of the 40 clinical isolates tested were positive for the bla SHV-ESBL point mutation when tested by the LDR-PCR method but negative when tested by the E test. In contrast to the E test or other molecular genetic tests, the LDR-PCR method is able to identify a single bacterium with a point mutation in a background of 100,000 wild-type (non-ESBL-producing) bacteria. Springer-Verlag Berlin Heidelberg, 2000 Niederhauser C. Institute of Microbiology, Kantonsspital Aarau, 5001 Aarau, Switzerland e-mail: Christoph.Niederhauser@KSA.ch, CH aut Kaempf L. Institute of Microbiology, Kantonsspital Aarau, 5001 Aarau, Switzerland e-mail: Christoph.Niederhauser@KSA.ch, CH aut Heinzer I. Institute of Microbiology, Kantonsspital Aarau, 5001 Aarau, Switzerland e-mail: Christoph.Niederhauser@KSA.ch, CH aut https://doi.org/10.1007/s100960000285 text/html Onlinezugriff via DOI 1 research-article jats NATIONALLICENCE 856 40 https://doi.org/10.1007/s100960000285 text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Niederhauser C. Institute of Microbiology, Kantonsspital Aarau, 5001 Aarau, Switzerland e-mail: Christoph.Niederhauser@KSA.ch, CH aut NATIONALLICENCE 700 1- Kaempf L. Institute of Microbiology, Kantonsspital Aarau, 5001 Aarau, Switzerland e-mail: Christoph.Niederhauser@KSA.ch, CH aut NATIONALLICENCE 700 1- Heinzer I. Institute of Microbiology, Kantonsspital Aarau, 5001 Aarau, Switzerland e-mail: Christoph.Niederhauser@KSA.ch, CH aut Metadata rights reserved Springer special CC-BY-NC licence nationallicence BK010053 XK010053 XK010000 NATIONALLICENCE NATIONALLICENCE NL-springer