caa a22 4500 475831438 CHVBK 20180406123841.0 cr unu---uuuuu 170329e20001001xx s 000 0 eng 10.1007/s100960000340 doi (NATIONALLICENCE)springer-10.1007/s100960000340 Assessment of Optimal Atmospheric Conditions for Growth of Helicobacter pylori [Elektronische Daten] [T.-H. Henriksen, A. Lia, R. Schøyen, T. Thoresen, A. Berstad]  The number and diameter of colonies of Helicobacter pylori isolates growing on agar plates were determined to compare five methods that produce a culture atmosphere. No catalyst was applied. No significant difference was found between two hydrogen-based kits that have a different capacity for production of H2. These hydrogen-based methods were significantly better than all others evaluated, including one kit that produces ascorbic acid that binds with oxygen. Growth was significantly improved when the atmosphere outside the plastic incubation jars was enriched with 10% CO2, but carbon dioxide enrichment alone (i.e., no reduction of the oxygen concentration) gave a very poor yield. The colony diameter was a sensitive and reliable measure of atmospheric conditions, as the mean intra- and interobserver difference between repeated readings was ≤0.1 mm for 82% and ≤0.2 mm for 95% of the isolates. Springer-Verlag Berlin Heidelberg, 2000 Henriksen T.-H Department of Microbiology, Vestfold Sentralsykehus, 3116 Tønsberg, Norway e-mail: thorhenrik.henriksen@vf.telia.no, Tel.: +47-33324662, Fax: +47-33343939, NO aut Lia A. Department of Microbiology, Vestfold Sentralsykehus, 3116 Tønsberg, Norway e-mail: thorhenrik.henriksen@vf.telia.no, Tel.: +47-33324662, Fax: +47-33343939, NO aut Schøyen R. Department of Microbiology, Vestfold Sentralsykehus, 3116 Tønsberg, Norway e-mail: thorhenrik.henriksen@vf.telia.no, Tel.: +47-33324662, Fax: +47-33343939, NO aut Thoresen T. Department of Microbiology, Vestfold Sentralsykehus, 3116 Tønsberg, Norway e-mail: thorhenrik.henriksen@vf.telia.no, Tel.: +47-33324662, Fax: +47-33343939, NO aut Berstad A. Department of Internal Medicine, Haukeland Sykehus, 5021 Bergen, Norway, NO aut https://doi.org/10.1007/s100960000340 text/html Onlinezugriff via DOI 1 research-article jats NATIONALLICENCE 856 40 https://doi.org/10.1007/s100960000340 text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Henriksen T.-H Department of Microbiology, Vestfold Sentralsykehus, 3116 Tønsberg, Norway e-mail: thorhenrik.henriksen@vf.telia.no, Tel.: +47-33324662, Fax: +47-33343939, NO aut NATIONALLICENCE 700 1- Lia A. Department of Microbiology, Vestfold Sentralsykehus, 3116 Tønsberg, Norway e-mail: thorhenrik.henriksen@vf.telia.no, Tel.: +47-33324662, Fax: +47-33343939, NO aut NATIONALLICENCE 700 1- Schøyen R. Department of Microbiology, Vestfold Sentralsykehus, 3116 Tønsberg, Norway e-mail: thorhenrik.henriksen@vf.telia.no, Tel.: +47-33324662, Fax: +47-33343939, NO aut NATIONALLICENCE 700 1- Thoresen T. Department of Microbiology, Vestfold Sentralsykehus, 3116 Tønsberg, Norway e-mail: thorhenrik.henriksen@vf.telia.no, Tel.: +47-33324662, Fax: +47-33343939, NO aut NATIONALLICENCE 700 1- Berstad A. Department of Internal Medicine, Haukeland Sykehus, 5021 Bergen, Norway, NO aut Metadata rights reserved Springer special CC-BY-NC licence nationallicence BK010053 XK010053 XK010000 NATIONALLICENCE NATIONALLICENCE NL-springer