caa a22 4500 475831578 CHVBK 20180406123841.0 cr unu---uuuuu 170329e20000801xx s 000 0 eng 10.1007/s100960000302 doi (NATIONALLICENCE)springer-10.1007/s100960000302 Nosocomial Spread of Hepatitis B Virus in Two Hemodialysis Units, Investigated by Restriction Fragment Length Polymorphism Analysis [Elektronische Daten] [L. De Castro, N. M. Araujo, R. R. Sabino, F. Alvarenga, C. F. T. Yoshida, S. A. Gomes]  A method for genotyping hepatitis B virus (HBV) strains, based on restriction fragment length polymorphism (RFLP) analysis of four different amplified fragments of the HBV genome, was used to investigate nosocomial infections that occurred in two Brazilian hemodialysis centers. Viral isolates from hepatitis B surface antigen (HBsAg)-positive serum samples from 27 hemodialysis patients and 39 HBV-positive unrelated control patients were grouped according to their RFLP patterns. Strains isolated from the control patients were divided into nine RFLP patterns: A1, A2, A3 (genotype A), D1, D2, D3, D4 (genotype D), F1, and F2 (genotype F). In hemodialysis unit A (Rio de Janeiro), 14 HBV isolates were grouped into five different RFLP patterns: A1, A2, A3, D3, and D4. Pattern A2, present at a relatively low prevalence (18%) in the control group, was observed in the majority (53%) of the hemodialysis patients. Notably, all five patients who seroconverted to HBsAg positivity in 1995 carried the strain A2. In hemodialysis unit B (state of São Paulo), where an outbreak of HBV infection occurred in 1996-1997, RFLP analysis showed that all 13 patients who seroconverted were infected with HBV isolates of genotype D. Coinfection with strain A1 was detected in seven of them. The results demonstrate the value of RFLP analysis in establishing common sources of infection in hemodialysis centers. Springer-Verlag Berlin Heidelberg, 2000 De Castro L. Departamento de Virologia, Instituto Oswaldo Cruz, FIOCRUZ, Av. Brasil 4365, 21045-900 Rio de Janeiro, RJ, Brazil e-mail: sagomes@gene.dbbm.fiocruz.br, BR aut Araujo N. M. Departamento de Virologia, Instituto Oswaldo Cruz, FIOCRUZ, Av. Brasil 4365, 21045-900 Rio de Janeiro, RJ, Brazil e-mail: sagomes@gene.dbbm.fiocruz.br, BR aut Sabino R. R. Departamento de Virologia, Instituto Oswaldo Cruz, FIOCRUZ, Av. Brasil 4365, 21045-900 Rio de Janeiro, RJ, Brazil e-mail: sagomes@gene.dbbm.fiocruz.br, BR aut Alvarenga F. Unidade de Hemodiálise, Hospital Evangélico, Rua Bom Pastor 295, 20521-060 Rio de Janeiro, RJ, Brazil, BR aut Yoshida C. F. T. Departamento de Virologia, Instituto Oswaldo Cruz, FIOCRUZ, Av. Brasil 4365, 21045-900 Rio de Janeiro, RJ, Brazil e-mail: sagomes@gene.dbbm.fiocruz.br, BR aut Gomes S. A. Departamento de Virologia, Instituto Oswaldo Cruz, FIOCRUZ, Av. Brasil 4365, 21045-900 Rio de Janeiro, RJ, Brazil e-mail: sagomes@gene.dbbm.fiocruz.br, BR aut https://doi.org/10.1007/s100960000302 text/html Onlinezugriff via DOI 1 research-article jats NATIONALLICENCE 856 40 https://doi.org/10.1007/s100960000302 text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- De Castro L. Departamento de Virologia, Instituto Oswaldo Cruz, FIOCRUZ, Av. Brasil 4365, 21045-900 Rio de Janeiro, RJ, Brazil e-mail: sagomes@gene.dbbm.fiocruz.br, BR aut NATIONALLICENCE 700 1- Araujo N. M. Departamento de Virologia, Instituto Oswaldo Cruz, FIOCRUZ, Av. Brasil 4365, 21045-900 Rio de Janeiro, RJ, Brazil e-mail: sagomes@gene.dbbm.fiocruz.br, BR aut NATIONALLICENCE 700 1- Sabino R. R. Departamento de Virologia, Instituto Oswaldo Cruz, FIOCRUZ, Av. Brasil 4365, 21045-900 Rio de Janeiro, RJ, Brazil e-mail: sagomes@gene.dbbm.fiocruz.br, BR aut NATIONALLICENCE 700 1- Alvarenga F. Unidade de Hemodiálise, Hospital Evangélico, Rua Bom Pastor 295, 20521-060 Rio de Janeiro, RJ, Brazil, BR aut NATIONALLICENCE 700 1- Yoshida C. F. T. Departamento de Virologia, Instituto Oswaldo Cruz, FIOCRUZ, Av. Brasil 4365, 21045-900 Rio de Janeiro, RJ, Brazil e-mail: sagomes@gene.dbbm.fiocruz.br, BR aut NATIONALLICENCE 700 1- Gomes S. A. Departamento de Virologia, Instituto Oswaldo Cruz, FIOCRUZ, Av. Brasil 4365, 21045-900 Rio de Janeiro, RJ, Brazil e-mail: sagomes@gene.dbbm.fiocruz.br, BR aut Metadata rights reserved Springer special CC-BY-NC licence nationallicence BK010053 XK010053 XK010000 NATIONALLICENCE NATIONALLICENCE NL-springer