caa a22 4500 475831608 CHVBK 20180406123841.0 cr unu---uuuuu 170329e20000801xx s 000 0 eng 10.1007/s100960000307 doi (NATIONALLICENCE)springer-10.1007/s100960000307 Strain Characterization of Candida parapsilosis Fungemia by Molecular Typing Methods [Elektronische Daten] [R. M. Zancopé-Oliveira, M. J. James, A. P. Derossi, J. L. M. Sampaio, M. M. Muniz, R. K. Li, A. S. A. Nascimento, J. M. Peralta, E. Reiss]  The present study used two molecular typing methods to investigate a cluster of eight cases of Candida parapsilosis fungemia in a hospital in Rio de Janeiro, Brazil. Candida parapsilosis is an important opportunistic pathogen that is frequently involved in outbreaks of nosocomial fungemia. Identification of a common source of infection and determination of genetic relatedness among the strains involved in outbreaks are important for infection control. Candida parapsilosis strains were isolated from the bloodstream of patients housed in an intensive-care unit (n=5) and in individual rooms (n=3). An additional strain of Candida parapsilosis was isolated from a hyperalimentation infusion flask, which was implicated by molecular typing to be the source of infection. All strains were identified using morphological and biochemical methods. The genetic relationship between patients' strains and the hyperalimentation infusion strain was assessed by electrophoretic karyotype (EK) analysis and random amplification of polymorphic DNA (RAPD). Both methods resulted in patterns that allowed differentiation of the isolates. Candida parapsilosis fungemia, in three of the eight patients, resulted from a common source of infection, as demonstrated by molecular typing methods. Image analysis of EK patterns indicated that these strains were closest to Candida parapsilosis Group II, a grouping that is a less frequent clinical isolate than the major Group I strains. Springer-Verlag Berlin Heidelberg, 2000 Zancopé-Oliveira R. M. Centro de Pesquisas Hospital Evandro Chagas, Fundação Oswaldo Cruz, Av. Brasil, 4365 CEP 21045-900 Rio de Janeiro, Brazil e-mail: zancope@fiocruz.br, BR aut James M. J. Centers for Disease Control and Prevention, 1600 Clifton Road N.E., Atlanta, GA 30333, USA, US aut Derossi A. P. Laboratório Lâmina, Rio de Janeiro, Brazil, BR aut Sampaio J. L. M. Laboratório Lâmina, Rio de Janeiro, Brazil, BR aut Muniz M. M. Centro de Pesquisas Hospital Evandro Chagas, Fundação Oswaldo Cruz, Av. Brasil, 4365 CEP 21045-900 Rio de Janeiro, Brazil e-mail: zancope@fiocruz.br, BR aut Li R. K. Centers for Disease Control and Prevention, 1600 Clifton Road N.E., Atlanta, GA 30333, USA, US aut Nascimento A. S. A. Instituto de Microbiologia da Universidade Federal do Rio de Janeiro, Brazil, BR aut Peralta J. M. Instituto de Microbiologia da Universidade Federal do Rio de Janeiro, Brazil, BR aut Reiss E. Centers for Disease Control and Prevention, 1600 Clifton Road N.E., Atlanta, GA 30333, USA, US aut https://doi.org/10.1007/s100960000307 text/html Onlinezugriff via DOI 1 research-article jats NATIONALLICENCE 856 40 https://doi.org/10.1007/s100960000307 text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Zancopé-Oliveira R. M. Centro de Pesquisas Hospital Evandro Chagas, Fundação Oswaldo Cruz, Av. Brasil, 4365 CEP 21045-900 Rio de Janeiro, Brazil e-mail: zancope@fiocruz.br, BR aut NATIONALLICENCE 700 1- James M. J. Centers for Disease Control and Prevention, 1600 Clifton Road N.E., Atlanta, GA 30333, USA, US aut NATIONALLICENCE 700 1- Derossi A. P. Laboratório Lâmina, Rio de Janeiro, Brazil, BR aut NATIONALLICENCE 700 1- Sampaio J. L. M. Laboratório Lâmina, Rio de Janeiro, Brazil, BR aut NATIONALLICENCE 700 1- Muniz M. M. Centro de Pesquisas Hospital Evandro Chagas, Fundação Oswaldo Cruz, Av. Brasil, 4365 CEP 21045-900 Rio de Janeiro, Brazil e-mail: zancope@fiocruz.br, BR aut NATIONALLICENCE 700 1- Li R. K. Centers for Disease Control and Prevention, 1600 Clifton Road N.E., Atlanta, GA 30333, USA, US aut NATIONALLICENCE 700 1- Nascimento A. S. A. Instituto de Microbiologia da Universidade Federal do Rio de Janeiro, Brazil, BR aut NATIONALLICENCE 700 1- Peralta J. M. Instituto de Microbiologia da Universidade Federal do Rio de Janeiro, Brazil, BR aut NATIONALLICENCE 700 1- Reiss E. Centers for Disease Control and Prevention, 1600 Clifton Road N.E., Atlanta, GA 30333, USA, US aut Metadata rights reserved Springer special CC-BY-NC licence nationallicence BK010053 XK010053 XK010000 NATIONALLICENCE NATIONALLICENCE NL-springer