caa a22 4500 475831748 CHVBK 20180406123841.0 cr unu---uuuuu 170329e20001201xx s 000 0 eng 10.1007/s100960000406 doi (NATIONALLICENCE)springer-10.1007/s100960000406 Screening for Antimicrobial Resistance in Normal Bacterial Flora of the Skin Using the Replica Plating Method [Elektronische Daten] [T. Paavilainen, M. Österblad, T. Leistevuo, P. Huovinen, P. Kotilainen]  The replica plating method was evaluated for detection of the antimicrobial resistance of normal bacterial flora of the skin and was compared with the results of a ten-colony method. If ≥10% of the colonies from the master plate grew on a plate containing an antibiotic, the sensitivity of replica plating was comparable to that of a ten-colony method for samples containing resistant bacteria. However, this method classified significantly more samples as resistant to all eight antibiotics tested if the detection breakpoint was lowered to ≥1% of the original colonies. Replica plating is an effective and practical tool for screening skin flora for resistance, also in samples with a low proportion of resistant strains. Springer-Verlag Berlin Heidelberg, 2000 Paavilainen T. Antimicrobial Research Laboratory, National Public Health Institute, PO Box 57, FIN-20521, Turku, Finland, FI aut Österblad M. Antimicrobial Research Laboratory, National Public Health Institute, PO Box 57, FIN-20521, Turku, Finland, FI aut Leistevuo T. Antimicrobial Research Laboratory, National Public Health Institute, PO Box 57, FIN-20521, Turku, Finland, FI aut Huovinen P. Antimicrobial Research Laboratory, National Public Health Institute, PO Box 57, FIN-20521, Turku, Finland, FI aut Kotilainen P. Department of Medicine, Division of Infectious Diseases, Turku University Central Hospital, Kiinamyllynkatu 4-8, 20520 Turku, Finland e-mail: pirkko.kotilainen@utu.fi Tel.: +358-2-2611611 Fax: +358-2-2612030, FI aut https://doi.org/10.1007/s100960000406 text/html Onlinezugriff via DOI 1 research-article jats NATIONALLICENCE 856 40 https://doi.org/10.1007/s100960000406 text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Paavilainen T. Antimicrobial Research Laboratory, National Public Health Institute, PO Box 57, FIN-20521, Turku, Finland, FI aut NATIONALLICENCE 700 1- Österblad M. Antimicrobial Research Laboratory, National Public Health Institute, PO Box 57, FIN-20521, Turku, Finland, FI aut NATIONALLICENCE 700 1- Leistevuo T. Antimicrobial Research Laboratory, National Public Health Institute, PO Box 57, FIN-20521, Turku, Finland, FI aut NATIONALLICENCE 700 1- Huovinen P. Antimicrobial Research Laboratory, National Public Health Institute, PO Box 57, FIN-20521, Turku, Finland, FI aut NATIONALLICENCE 700 1- Kotilainen P. Department of Medicine, Division of Infectious Diseases, Turku University Central Hospital, Kiinamyllynkatu 4-8, 20520 Turku, Finland e-mail: pirkko.kotilainen@utu.fi Tel.: +358-2-2611611 Fax: +358-2-2612030, FI aut Metadata rights reserved Springer special CC-BY-NC licence nationallicence BK010053 XK010053 XK010000 NATIONALLICENCE NATIONALLICENCE NL-springer