caa a22 4500 475831799 CHVBK 20180406123842.0 cr unu---uuuuu 170329e20001201xx s 000 0 eng 10.1007/s100960000397 doi (NATIONALLICENCE)springer-10.1007/s100960000397 Detection of Seroconversion and Persistence of Chlamydia trachomatis Antibodies in Five Different Serological Tests [Elektronische Daten] [A. Clad, H. M. Freidank, M. Kunze, U. Schnoeckel, S. Hofmeier, U. Flecken, E. E. Petersen]  Microimmunofluorescence (MIF), a Chlamydia trachomatis species-specific enzyme immunoassay incorporating lipopolysaccharide-extracted Chlamydia trachomatis L2 elementary bodies, two different synthetic peptide-based species-specific tests, and a recombinant lipopolysaccharide genus-specific test were performed on multiple follow-up sera (n=104 total) from 16 women with Chlamydia trachomatis-positive cervical swabs. These women included five with IgG seroconversions, five with Chlamydia trachomatis reinfections after initial therapy, and six with serologic follow-up of more than 6 years after antibiotic therapy. Of all the tests employed in this study, MIF IgG reverted earliest to negative titers, while MIF IgA was the least sensitive. The lipopolysaccharide-extracted elementary body enzyme immunoassay exhibited the closest correlation with the MIF test. The highest test sensitivity was observed in one of the synthetic peptide-based tests, which detected earliest seroconversions and longest IgG persistence. The other synthetic peptide-based test gave false-negative results in 2 of 16 women and did not detect seroconversion earlier than the MIF test. Seroconversion and persistence of genus-specific IgG - cross-reactivity with Chlamydia pneumoniae- against lipopolysaccharide were similar to species-specific IgG. A significant serologic response to reinfection was observed only in women with signs of pelvic inflammatory disease. Species-specific tests of high sensitivity and reproducibility are best suited for gynecological diagnostic purposes. Springer-Verlag Berlin Heidelberg, 2000 Clad A. Frauenklinik der Universität Freiburg, Sektion Infektiologie, Hugstetterstrasse 55, 79106 Freiburg, Germany e-mail: ACLAD@frk1.ukl.uni-freiburg.de Tel: +49-761-2703002 Fax: +49-761-2703060, DE aut Freidank H. M. Institut für Medizinische Mikrobiologie und Hygiene, Hermann-Herderstrasse 11, 79104 Freiburg, Germany, DE aut Kunze M. Institut für Medizinische Mikrobiologie und Hygiene, Hermann-Herderstrasse 11, 79104 Freiburg, Germany, DE aut Schnoeckel U. Frauenklinik der Universität Freiburg, Sektion Infektiologie, Hugstetterstrasse 55, 79106 Freiburg, Germany e-mail: ACLAD@frk1.ukl.uni-freiburg.de Tel: +49-761-2703002 Fax: +49-761-2703060, DE aut Hofmeier S. Frauenklinik der Universität Freiburg, Sektion Infektiologie, Hugstetterstrasse 55, 79106 Freiburg, Germany e-mail: ACLAD@frk1.ukl.uni-freiburg.de Tel: +49-761-2703002 Fax: +49-761-2703060, DE aut Flecken U. Frauenklinik der Universität Freiburg, Sektion Infektiologie, Hugstetterstrasse 55, 79106 Freiburg, Germany e-mail: ACLAD@frk1.ukl.uni-freiburg.de Tel: +49-761-2703002 Fax: +49-761-2703060, DE aut Petersen E. E. Frauenklinik der Universität Freiburg, Sektion Infektiologie, Hugstetterstrasse 55, 79106 Freiburg, Germany e-mail: ACLAD@frk1.ukl.uni-freiburg.de Tel: +49-761-2703002 Fax: +49-761-2703060, DE aut https://doi.org/10.1007/s100960000397 text/html Onlinezugriff via DOI 1 research-article jats NATIONALLICENCE 856 40 https://doi.org/10.1007/s100960000397 text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Clad A. Frauenklinik der Universität Freiburg, Sektion Infektiologie, Hugstetterstrasse 55, 79106 Freiburg, Germany e-mail: ACLAD@frk1.ukl.uni-freiburg.de Tel: +49-761-2703002 Fax: +49-761-2703060, DE aut NATIONALLICENCE 700 1- Freidank H. M. Institut für Medizinische Mikrobiologie und Hygiene, Hermann-Herderstrasse 11, 79104 Freiburg, Germany, DE aut NATIONALLICENCE 700 1- Kunze M. Institut für Medizinische Mikrobiologie und Hygiene, Hermann-Herderstrasse 11, 79104 Freiburg, Germany, DE aut NATIONALLICENCE 700 1- Schnoeckel U. Frauenklinik der Universität Freiburg, Sektion Infektiologie, Hugstetterstrasse 55, 79106 Freiburg, Germany e-mail: ACLAD@frk1.ukl.uni-freiburg.de Tel: +49-761-2703002 Fax: +49-761-2703060, DE aut NATIONALLICENCE 700 1- Hofmeier S. Frauenklinik der Universität Freiburg, Sektion Infektiologie, Hugstetterstrasse 55, 79106 Freiburg, Germany e-mail: ACLAD@frk1.ukl.uni-freiburg.de Tel: +49-761-2703002 Fax: +49-761-2703060, DE aut NATIONALLICENCE 700 1- Flecken U. Frauenklinik der Universität Freiburg, Sektion Infektiologie, Hugstetterstrasse 55, 79106 Freiburg, Germany e-mail: ACLAD@frk1.ukl.uni-freiburg.de Tel: +49-761-2703002 Fax: +49-761-2703060, DE aut NATIONALLICENCE 700 1- Petersen E. E. Frauenklinik der Universität Freiburg, Sektion Infektiologie, Hugstetterstrasse 55, 79106 Freiburg, Germany e-mail: ACLAD@frk1.ukl.uni-freiburg.de Tel: +49-761-2703002 Fax: +49-761-2703060, DE aut Metadata rights reserved Springer special CC-BY-NC licence nationallicence BK010053 XK010053 XK010000 NATIONALLICENCE NATIONALLICENCE NL-springer