caa a22 4500 475831802 CHVBK 20180406123842.0 cr unu---uuuuu 170329e20001201xx s 000 0 eng 10.1007/s100960000407 doi (NATIONALLICENCE)springer-10.1007/s100960000407 Chronic Cystitis Caused by Corynebacterium urealyticum Detected by Polymerase Chain Reaction [Elektronische Daten] [A. M. Simoons-Smit, P. H. M. Savelkoul, D. W. W. Newling, C. M. J. Vandenbroucke-Grauls]  The case of a 73-year-old man with chronic cystitis due to Corynebacterium urealyticum was complicated by hematuria and urinary stone formation. The diagnosis was based on an amplification product obtained using polymerase chain reaction for mycobacterial species on urine and a bladder biopsy specimen. A specific 212 bp amplification fragment that did not hybridize with a Mycobacterium-specific probe was recognized. Sequence analysis of the fragment revealed Corynebacterium urealyticum. Routine urine cultures were negative, but prolonged culture on sheep blood agar led to the isolation and identification of Corynebacterium urealyticum. Identification was confirmed by polymerase chain reaction on the colonies. The patient was treated successfully with vancomycin. Integration of molecular laboratory diagnostics with conventional microbiology and pathology was synergistic for the diagnosis. Springer-Verlag Berlin Heidelberg, 2000 Simoons-Smit A. M. Department of Medical Microbiology and Infection Control, University Hospital Vrije Universiteit, PO Box 7057, 1007 MB Amsterdam, The Netherlands e-mail: am.simoons@azvu.nl Tel.: +31-2-4440484 Fax: +31-20-4440473, NL aut Savelkoul P. H. M. Department of Medical Microbiology and Infection Control, University Hospital Vrije Universiteit, PO Box 7057, 1007 MB Amsterdam, The Netherlands e-mail: am.simoons@azvu.nl Tel.: +31-2-4440484 Fax: +31-20-4440473, NL aut Newling D. W. W. Department of Urology, University Hospital Vrije Universiteit, PO Box 7057, 1007 MB Amsterdam, The Netherlands, NL aut Vandenbroucke-Grauls C. M. J. Department of Medical Microbiology and Infection Control, University Hospital Vrije Universiteit, PO Box 7057, 1007 MB Amsterdam, The Netherlands e-mail: am.simoons@azvu.nl Tel.: +31-2-4440484 Fax: +31-20-4440473, NL aut https://doi.org/10.1007/s100960000407 text/html Onlinezugriff via DOI 1 research-article jats NATIONALLICENCE 856 40 https://doi.org/10.1007/s100960000407 text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Simoons-Smit A. M. Department of Medical Microbiology and Infection Control, University Hospital Vrije Universiteit, PO Box 7057, 1007 MB Amsterdam, The Netherlands e-mail: am.simoons@azvu.nl Tel.: +31-2-4440484 Fax: +31-20-4440473, NL aut NATIONALLICENCE 700 1- Savelkoul P. H. M. Department of Medical Microbiology and Infection Control, University Hospital Vrije Universiteit, PO Box 7057, 1007 MB Amsterdam, The Netherlands e-mail: am.simoons@azvu.nl Tel.: +31-2-4440484 Fax: +31-20-4440473, NL aut NATIONALLICENCE 700 1- Newling D. W. W. Department of Urology, University Hospital Vrije Universiteit, PO Box 7057, 1007 MB Amsterdam, The Netherlands, NL aut NATIONALLICENCE 700 1- Vandenbroucke-Grauls C. M. J. Department of Medical Microbiology and Infection Control, University Hospital Vrije Universiteit, PO Box 7057, 1007 MB Amsterdam, The Netherlands e-mail: am.simoons@azvu.nl Tel.: +31-2-4440484 Fax: +31-20-4440473, NL aut Metadata rights reserved Springer special CC-BY-NC licence nationallicence BK010053 XK010053 XK010000 NATIONALLICENCE NATIONALLICENCE NL-springer