caa a22 4500 47583187X CHVBK 20180406123842.0 cr unu---uuuuu 170329e20000401xx s 000 0 eng 10.1007/s100960050457 doi (NATIONALLICENCE)springer-10.1007/s100960050457 Evaluation of the Inactivation of Infectious Herpes Simplex Virus by Host-Defense Peptides [Elektronische Daten] [B. Yasin, M. Pang, J. S. Turner, Y. Cho, N-N. Dinh, A. J. Waring, R. I. Lehrer, E. A. Wagar]  A 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide microplate assay was adapted to screen for the ability of 20 host-defense peptides to inactivate herpes simplex virus type 1 and type 2. The procedure required minimal amounts of material, was reproducible, and was confirmed with standard antiviral testing techniques. In screening tests, with the exception of melittin, a highly cytotoxic and hemolytic peptide found in bee venom, the α-helical peptides in our test panel (magainins, cecropins, clavanins, and LL-37) caused little viral inactivation. Several β-sheet peptides (defensins, tachyplesin, and protegrins) inactivated one or both viruses, sometimes with remarkable selectivity. Two peptides were identified as having antiviral activity against both viruses, indolicidin (a tryptophan-rich peptide from bovine neutrophils) and brevinin-1 (a peptide found in frog skin). The antiviral activity of these two peptides was confirmed with standard antiviral assays. Interestingly, the antiviral activity of brevinin-1 was maintained after reduction and carboxamidomethylation, procedures that abolished its otherwise prominent hemolytic and cytotoxic effects. Springer-Verlag Berlin Heidelberg, 2000 Yasin B. Department of Pathology and Laboratory Medicine, UCLA School of Medicine, CHS 1P-244, Los Angeles, CA 90095-1731, USA e-mail: ewagar@mednet.ucla.edu, US aut Pang M. Department of Pathology and Laboratory Medicine, UCLA School of Medicine, CHS 1P-244, Los Angeles, CA 90095-1731, USA e-mail: ewagar@mednet.ucla.edu, US aut Turner J. S. Department of Medicine, UCLA School of Medicine, CHS 37-062, Los Angeles, CA 90095-1690, USA, US aut Cho Y. Department of Medicine, UCLA School of Medicine, CHS 37-062, Los Angeles, CA 90095-1690, USA, US aut Dinh N-N Department of Medicine, UCLA School of Medicine, CHS 37-062, Los Angeles, CA 90095-1690, USA, US aut Waring A. J. Department of Medicine, UCLA School of Medicine, CHS 37-062, Los Angeles, CA 90095-1690, USA, US aut Lehrer R. I. Department of Medicine, UCLA School of Medicine, CHS 37-062, Los Angeles, CA 90095-1690, USA, US aut Wagar E. A. Department of Pathology and Laboratory Medicine, UCLA School of Medicine, CHS 1P-244, Los Angeles, CA 90095-1731, USA e-mail: ewagar@mednet.ucla.edu, US aut https://doi.org/10.1007/s100960050457 text/html Onlinezugriff via DOI 1 research-article jats NATIONALLICENCE 856 40 https://doi.org/10.1007/s100960050457 text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Yasin B. Department of Pathology and Laboratory Medicine, UCLA School of Medicine, CHS 1P-244, Los Angeles, CA 90095-1731, USA e-mail: ewagar@mednet.ucla.edu, US aut NATIONALLICENCE 700 1- Pang M. Department of Pathology and Laboratory Medicine, UCLA School of Medicine, CHS 1P-244, Los Angeles, CA 90095-1731, USA e-mail: ewagar@mednet.ucla.edu, US aut NATIONALLICENCE 700 1- Turner J. S. Department of Medicine, UCLA School of Medicine, CHS 37-062, Los Angeles, CA 90095-1690, USA, US aut NATIONALLICENCE 700 1- Cho Y. Department of Medicine, UCLA School of Medicine, CHS 37-062, Los Angeles, CA 90095-1690, USA, US aut NATIONALLICENCE 700 1- Dinh N-N Department of Medicine, UCLA School of Medicine, CHS 37-062, Los Angeles, CA 90095-1690, USA, US aut NATIONALLICENCE 700 1- Waring A. J. Department of Medicine, UCLA School of Medicine, CHS 37-062, Los Angeles, CA 90095-1690, USA, US aut NATIONALLICENCE 700 1- Lehrer R. I. Department of Medicine, UCLA School of Medicine, CHS 37-062, Los Angeles, CA 90095-1690, USA, US aut NATIONALLICENCE 700 1- Wagar E. A. Department of Pathology and Laboratory Medicine, UCLA School of Medicine, CHS 1P-244, Los Angeles, CA 90095-1731, USA e-mail: ewagar@mednet.ucla.edu, US aut Metadata rights reserved Springer special CC-BY-NC licence nationallicence BK010053 XK010053 XK010000 NATIONALLICENCE NATIONALLICENCE NL-springer