caa a22 4500 475832035 CHVBK 20180406123842.0 cr unu---uuuuu 170329e20001101xx s 000 0 eng 10.1007/s100960000359 doi (NATIONALLICENCE)springer-10.1007/s100960000359 Assessment of Intercentre Reproducibility and Epidemiological Concordance of Legionella pneumophila Serogroup 1 Genotyping by Amplified Fragment Length Polymorphism Analysis [Elektronische Daten] [N. K. Fry, J. M. Bangsborg, S. Bernander, J. Etienne, B. Forsblom, V. Gaia, P. Hasenberger, D. Lindsay, A. Papoutsi, C. Pelaz, M. Struelens, S. A. Uldum, P. Visca, T. G. Harrison]  The aims of this work were to assess (i) the intercentre reproducibility and epidemiological concordance of amplified fragment length polymorphism analysis for epidemiological typing of Legionella pneumophila serogroup 1, and (ii) the suitability of the method for standardisation and implementation by members of the European Working Group on Legionella Infections. Fifty coded isolates comprising two panels of well-characterised strains, a "reproducibility” panel (n=20) and an "epidemiologically related” panel (n=30), were sent to 13 centres in 12 European countries. Analysis was undertaken in each centre following a previously determined standard protocol. Results were analysed by the participants, using gel analysis software where available, and submitted to the coordinating centre. The coordinating centre reanalysed all results visually and selected data-sets with gel analysis software. Data analysis by participants yielded reproducibility (R) values of 0.20-1.00 and epidemiological concordance (E) values of 0.11-1.00, with 6 to 34 types. Following visual analysis by the coordinating centre, R=0.78-1.00, and E=0.67-1.00, with 10-20 types. Analysis of three data-sets by the coordinating centre using gel analysis software yielded R=1.00 and E=1.00, with 12, 13 or 14 types. This method can be used as a simple, rapid screening tool for epidemiological typing of isolates of Legionella pneumophila serogroup 1. Results demonstrate that the method can be highly reproducible (R=1.00) and epidemiologically concordant (E=1.00), with good discrimination. The electropherograms generated are amenable to computer-aided analysis, but strict adherence to a previously defined laboratory protocol is required. Following designation of representative type strains and patterns, this method will be adopted by the European Working Group on Legionella Infections as the first internationally standardised typing method for use in the investigation of travel-associated Legionella infections. Springer-Verlag Berlin Heidelberg, 2000 Fry N. K. Respiratory and Systemic Infection Laboratory, Public Health Laboratory Service Central Public Health Laboratory, 61 Colindale Avenue, London NW9 5HT, UK e-mail: nfry@phls.nhs.uk, GB aut Bangsborg J. M. Department of Clinical Microbiology, Rigshospitalet, Copenhagen, Denmark, DK aut Bernander S. Department of Clinical Microbiology, Karolinska Sjukhuset, Stockholm, Sweden, SE aut Etienne J. Centre National de Référence des Legionella et des Staphylocoques, Lyon, France, FR aut Forsblom B. National Public Health Institute, Legionella Reference Laboratory, Helsinki, Finland, FI aut Gaia V. Istituto Cantonale Batteriosierologico, Lugano, Switzerland, CH aut Hasenberger P. Federal Public Health Laboratory, Vienna, Austria, AT aut Lindsay D. Scottish Legionella Reference Laboratory, Stobhill Hospital, Glasgow, Scotland, GB aut Papoutsi A. Department of Microbiology, School of Medicine, Aristotelian University of Thessaloniki, Thessaloniki, Greece, GR aut Pelaz C. Centro Nacional de Microbiologia, Majadahonda, Madrid, Spain, ES aut Struelens M. Service de Microbiologie, Laboratoire de Référence des Legionella, Hopital Erasme, Brussels, Belgium, BE aut Uldum S. A. Department of Respiratory Infections, Meningitis and Sexually Transmitted Diseases, Statens Serum Institut, Copenhagen, Denmark, DK aut Visca P. Unit of Special Pathogens, Istituto Superiore di Sanità, Rome, Italy Present address: Department of Biology, University of Rome III and Molecular Microbiology Unit, IRCCS "Lazzaro Spallanzani”, Rome, Italy, IT aut Harrison T. G. Respiratory and Systemic Infection Laboratory, Public Health Laboratory Service Central Public Health Laboratory, 61 Colindale Avenue, London NW9 5HT, UK e-mail: nfry@phls.nhs.uk, GB aut https://doi.org/10.1007/s100960000359 text/html Onlinezugriff via DOI 1 research-article jats NATIONALLICENCE 856 40 https://doi.org/10.1007/s100960000359 text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Fry N. K. Respiratory and Systemic Infection Laboratory, Public Health Laboratory Service Central Public Health Laboratory, 61 Colindale Avenue, London NW9 5HT, UK e-mail: nfry@phls.nhs.uk, GB aut NATIONALLICENCE 700 1- Bangsborg J. M. Department of Clinical Microbiology, Rigshospitalet, Copenhagen, Denmark, DK aut NATIONALLICENCE 700 1- Bernander S. Department of Clinical Microbiology, Karolinska Sjukhuset, Stockholm, Sweden, SE aut NATIONALLICENCE 700 1- Etienne J. Centre National de Référence des Legionella et des Staphylocoques, Lyon, France, FR aut NATIONALLICENCE 700 1- Forsblom B. National Public Health Institute, Legionella Reference Laboratory, Helsinki, Finland, FI aut NATIONALLICENCE 700 1- Gaia V. Istituto Cantonale Batteriosierologico, Lugano, Switzerland, CH aut NATIONALLICENCE 700 1- Hasenberger P. Federal Public Health Laboratory, Vienna, Austria, AT aut NATIONALLICENCE 700 1- Lindsay D. Scottish Legionella Reference Laboratory, Stobhill Hospital, Glasgow, Scotland, GB aut NATIONALLICENCE 700 1- Papoutsi A. Department of Microbiology, School of Medicine, Aristotelian University of Thessaloniki, Thessaloniki, Greece, GR aut NATIONALLICENCE 700 1- Pelaz C. Centro Nacional de Microbiologia, Majadahonda, Madrid, Spain, ES aut NATIONALLICENCE 700 1- Struelens M. Service de Microbiologie, Laboratoire de Référence des Legionella, Hopital Erasme, Brussels, Belgium, BE aut NATIONALLICENCE 700 1- Uldum S. A. Department of Respiratory Infections, Meningitis and Sexually Transmitted Diseases, Statens Serum Institut, Copenhagen, Denmark, DK aut NATIONALLICENCE 700 1- Visca P. Unit of Special Pathogens, Istituto Superiore di Sanità, Rome, Italy Present address: Department of Biology, University of Rome III and Molecular Microbiology Unit, IRCCS "Lazzaro Spallanzani”, Rome, Italy, IT aut NATIONALLICENCE 700 1- Harrison T. G. Respiratory and Systemic Infection Laboratory, Public Health Laboratory Service Central Public Health Laboratory, 61 Colindale Avenue, London NW9 5HT, UK e-mail: nfry@phls.nhs.uk, GB aut Metadata rights reserved Springer special CC-BY-NC licence nationallicence BK010053 XK010053 XK010000 NATIONALLICENCE NATIONALLICENCE NL-springer