caa a22 4500 475832051 CHVBK 20180406123842.0 cr unu---uuuuu 170329e20001101xx s 000 0 eng 10.1007/PL00011229 doi (NATIONALLICENCE)springer-10.1007/PL00011229 Macrolide Resistance Phenotypes and Genotypes in French Clinical Isolates of Streptococcus pneumoniae [Elektronische Daten] [P. Angot, M. Vergnaud, M. Auzou, R. Leclercq]  The aim of this study was to analyze the mechanisms of macrolide resistance in French clinical isolates of Streptococcus pneumoniae. A total of 838 strains of pneumococci were isolated in 1997 in Normandy, a region of western France, by 19 microbiology laboratories. Fifty-three percent had displayed diminished susceptibility to penicillin G and 50% were resistant to erythromycin. From this collection, 92 penicillin-intermediate or -resistant and 18 penicillin-susceptible strains resistant to erythromycin were studied. The presence of erm genes coding for ribosomal methylases and of mefE-like genes responsible for macrolide efflux was screened by a multiplex polymerase chain reaction and confirmed by DNA/DNA hybridization. Of the 110 strains studied, 108 were cross-resistant to erythromycin, spiramycin and clindamycin, including 105 strains containing ermB-related genes and three strains that contained a combination of ermB- and mefE-related genes. Two strains apparently susceptible to clindamycin but resistant to spiramycin also contained ermB-related genes. No strain was resistant to erythromycin alone or contained only a mef-like gene. Therefore, resistance to erythromycin is mostly related to ribosomal methylation in this region of France. Springer-Verlag Berlin Heidelberg, 2000 Angot P. Service de Microbiologie, CHU Côte de Nacre, Avenue Côte de Nacre, 14033 Caen, France e-mail: leclercq-r@chu-caen.fr, FR aut Vergnaud M. Service de Microbiologie, CHU Côte de Nacre, Avenue Côte de Nacre, 14033 Caen, France e-mail: leclercq-r@chu-caen.fr, FR aut Auzou M. Service de Microbiologie, CHU Côte de Nacre, Avenue Côte de Nacre, 14033 Caen, France e-mail: leclercq-r@chu-caen.fr, FR aut Leclercq R. Service de Microbiologie, CHU Côte de Nacre, Avenue Côte de Nacre, 14033 Caen, France e-mail: leclercq-r@chu-caen.fr, FR aut https://doi.org/10.1007/PL00011229 text/html Onlinezugriff via DOI 1 research-article jats NATIONALLICENCE 856 40 https://doi.org/10.1007/PL00011229 text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Angot P. Service de Microbiologie, CHU Côte de Nacre, Avenue Côte de Nacre, 14033 Caen, France e-mail: leclercq-r@chu-caen.fr, FR aut NATIONALLICENCE 700 1- Vergnaud M. Service de Microbiologie, CHU Côte de Nacre, Avenue Côte de Nacre, 14033 Caen, France e-mail: leclercq-r@chu-caen.fr, FR aut NATIONALLICENCE 700 1- Auzou M. Service de Microbiologie, CHU Côte de Nacre, Avenue Côte de Nacre, 14033 Caen, France e-mail: leclercq-r@chu-caen.fr, FR aut NATIONALLICENCE 700 1- Leclercq R. Service de Microbiologie, CHU Côte de Nacre, Avenue Côte de Nacre, 14033 Caen, France e-mail: leclercq-r@chu-caen.fr, FR aut Metadata rights reserved Springer special CC-BY-NC licence nationallicence BK010053 XK010053 XK010000 NATIONALLICENCE NATIONALLICENCE NL-springer