caa a22 4500 475832132 CHVBK 20180406123843.0 cr unu---uuuuu 170329e20001101xx s 000 0 eng 10.1007/s100960000368 doi (NATIONALLICENCE)springer-10.1007/s100960000368 Evaluation of a New Commercial Immunoassay >for Rapid Detection of Campylobacter jejuni in Stool Samples [Elektronische Daten] [H. P. Endtz, C. W. Ang, N. van den Braak, A. Luijendijk, B. C. Jacobs, P. de Man, J.M. van Duin, A. van Belkum, H. A. Verbrugh]  In order to evaluate a new commercial enzyme immunoassay (ProspecT Campylobacter Microplate Assay; Alexon-Trend, USA) for the detection of Campylobacter jejuni and Campylobacter coli in stool samples, 30 faecal specimens known to be culture-positive for Campylobacter jejuni were tested with the new assay. The detection limit was approximately 3×106/ml in faecal suspensions. The sensitivity relative to culture was 80% (24/30). All of the 24 positive samples, except for one, remained positive after being stored at -20  °C for 60 days. The specificity of the test was 100%. Interestingly, 6 of 11 additional Campylobacter jejuni culture-positive samples that had been obtained from patients with Guillain-Barré syndrome and stored at -20  °C for periods of up to 5 years tested positive in the assay. The performance of the assay indicates that it has potential value for use in future early intervention studies. Springer-Verlag Berlin Heidelberg, 2000 Endtz H. P. Department of Medical Microbiology & Infectious Diseases, Erasmus University Medical Centre Rotterdam, Dr. Molewaterplain 40, 3015 GD Rotterdam, The Netherlands e-mail: endtz@bacl.azr.nl, NL aut Ang C. W. Department of Neurology, Erasmus University Medical Centre Rotterdam, The Netherlands, NL aut van den Braak N. Department of Medical Microbiology & Infectious Diseases, Erasmus University Medical Centre Rotterdam, Dr. Molewaterplain 40, 3015 GD Rotterdam, The Netherlands e-mail: endtz@bacl.azr.nl, NL aut Luijendijk A. Department of Medical Microbiology & Infectious Diseases, Erasmus University Medical Centre Rotterdam, Dr. Molewaterplain 40, 3015 GD Rotterdam, The Netherlands e-mail: endtz@bacl.azr.nl, NL aut Jacobs B. C. Department of Neurology, Erasmus University Medical Centre Rotterdam, The Netherlands, NL aut de Man P. Department of Medical Microbiology, St. Franciscus Hospital, Rotterdam, The Netherlands, NL aut van Duin J.M. Department of Medical Microbiology, Zuiderziekenhuis, Rotterdam, The Netherlands, NL aut van Belkum A. Department of Medical Microbiology & Infectious Diseases, Erasmus University Medical Centre Rotterdam, Dr. Molewaterplain 40, 3015 GD Rotterdam, The Netherlands e-mail: endtz@bacl.azr.nl, NL aut Verbrugh H. A. Department of Medical Microbiology & Infectious Diseases, Erasmus University Medical Centre Rotterdam, Dr. Molewaterplain 40, 3015 GD Rotterdam, The Netherlands e-mail: endtz@bacl.azr.nl, NL aut https://doi.org/10.1007/s100960000368 text/html Onlinezugriff via DOI 1 research-article jats NATIONALLICENCE 856 40 https://doi.org/10.1007/s100960000368 text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Endtz H. P. Department of Medical Microbiology & Infectious Diseases, Erasmus University Medical Centre Rotterdam, Dr. Molewaterplain 40, 3015 GD Rotterdam, The Netherlands e-mail: endtz@bacl.azr.nl, NL aut NATIONALLICENCE 700 1- Ang C. W. Department of Neurology, Erasmus University Medical Centre Rotterdam, The Netherlands, NL aut NATIONALLICENCE 700 1- van den Braak N. Department of Medical Microbiology & Infectious Diseases, Erasmus University Medical Centre Rotterdam, Dr. Molewaterplain 40, 3015 GD Rotterdam, The Netherlands e-mail: endtz@bacl.azr.nl, NL aut NATIONALLICENCE 700 1- Luijendijk A. Department of Medical Microbiology & Infectious Diseases, Erasmus University Medical Centre Rotterdam, Dr. Molewaterplain 40, 3015 GD Rotterdam, The Netherlands e-mail: endtz@bacl.azr.nl, NL aut NATIONALLICENCE 700 1- Jacobs B. C. Department of Neurology, Erasmus University Medical Centre Rotterdam, The Netherlands, NL aut NATIONALLICENCE 700 1- de Man P. Department of Medical Microbiology, St. Franciscus Hospital, Rotterdam, The Netherlands, NL aut NATIONALLICENCE 700 1- van Duin J.M. Department of Medical Microbiology, Zuiderziekenhuis, Rotterdam, The Netherlands, NL aut NATIONALLICENCE 700 1- van Belkum A. Department of Medical Microbiology & Infectious Diseases, Erasmus University Medical Centre Rotterdam, Dr. Molewaterplain 40, 3015 GD Rotterdam, The Netherlands e-mail: endtz@bacl.azr.nl, NL aut NATIONALLICENCE 700 1- Verbrugh H. A. Department of Medical Microbiology & Infectious Diseases, Erasmus University Medical Centre Rotterdam, Dr. Molewaterplain 40, 3015 GD Rotterdam, The Netherlands e-mail: endtz@bacl.azr.nl, NL aut Metadata rights reserved Springer special CC-BY-NC licence nationallicence BK010053 XK010053 XK010000 NATIONALLICENCE NATIONALLICENCE NL-springer